TIE-2 Protein, Human (HEK293, His)

TIE-2 is a tyrosine protein kinase that serves as a cell surface receptor for ANGPT1, ANGPT2, and ANGPT4 and is critical for angiogenesis, endothelial cell processes, and vascular stability. In addition to embryonic vasculogenesis, TIE-2 also affects postnatal hematopoiesis, regulating angiogenesis depending on the situation. TIE-2 Protein, Human (HEK293, His) is the recombinant human-derived TIE-2 protein, expressed by HEK293 , with C-6*His labeled tag.

TIE-2 protein, a tyrosine-protein kinase, acts as a cell-surface receptor for ANGPT1, ANGPT2, and ANGPT4, orchestrating a comprehensive range of processes critical for angiogenesis, endothelial cell survival, proliferation, migration, adhesion, and cell spreading, as well as the maintenance of vascular quiescence. Beyond its essential role in embryonic angiogenesis and heart development, TIE-2 plays a vital role in post-natal hematopoiesis. Its function post-birth involves context-dependent activation or inhibition of angiogenesis. In quiescent vessels, ANGPT1 oligomers recruit TIE-2 to cell-cell contacts, fostering complex formation with neighboring TIE-2 molecules and preferential activation of the phosphatidylinositol 3-kinase and AKT1 signaling cascades, leading to vascular stability. Conversely, in migrating endothelial cells lacking cell-cell adhesions, ANGPT1 recruits TIE-2 to contacts with the extracellular matrix, activating focal adhesion complexes, PTK2/FAK, and downstream kinases MAPK1/ERK2 and MAPK3/ERK1, stimulating sprouting angiogenesis. ANGPT1-triggered TIE-2 signaling involves receptor dimerization and autophosphorylation at specific tyrosine residues, serving as binding sites for scaffold proteins and effectors. Modulation by ANGPT2, which competes for the same binding site, and formation of heterodimers with TIE1, as well as proteolytic processing yielding a soluble extracellular domain, further regulate TIE-2 signaling. The soluble extracellular domain functions as a decoy receptor for angiopoietins, influencing signaling dynamics. TIE-2 phosphorylates DOK2, GRB7, GRB14, PIK3R1, SHC1, and TIE1, underscoring its intricate role in finely tuning a myriad of cellular responses.

Human

HEK293

C-6*His

NP_000450.2 (A23-K745)

7010  [NCBI]

Partial

AMDLILINSLPLVSDAETSLTCIASGWRPHEPITIGRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGEAIRIRTMKMRQQASFLPATLTMTVDKGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPECNHLCTACMNNGVCHEDTGECICPPGFMGRTCEKACELHTFGRTCKERCSGQEGCKSYVFCLPDPYGCSCATGWKGLQCNEACHPGFYGPDCKLRCSCNNGEMCDRFQGCLCSPGWQGLQCEREGIQRMTPKIVDLPDHIEVNSGKFNPICKASGWPLPTNEEMTLVKPDGTVLHPKDFNHTDHFSVAIFTIHRILPPDSGVWVCSVNTVAGMVEKPFNISVKVLPKPLNAPNVIDTGHNFAVINISSEPYFGDGPIKSKKLLYKPVNHYEAWQHIQVTNEIVTLNYLEPRTEYELCVQLVRRGEGGEGHPGPVRRFTTASIGLPPPRGLNLLPKSQTTLNLTWQPIFPSSEDDFYVEVERRSVQKSDQQNIKVPGNLTSVLLNNLHPREQYVVRARVNTKAQGEWSEDLTAWTLSDILPPQPENIKISNITHSSAVISWTILDGYSISSITIRYKVQGKNEDQHVDVKIKNATITQYQLKGLEPETAYQVDIFAENNIGSSNPAFSHELVTLPESQAPADLGGGK

Approximately 100-120 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.