ANGPT1/Angiopoietin-1, Rat (P. pastoris, His)

In rats, ANGPT1 (Angiopoietin-1) activates the TIE2 receptor, inducing tyrosine phosphorylation and influencing endothelial developmental processes distinct from VEGF. ANGPT1 plays a crucial role in mediating reciprocal interactions between endothelium, matrix, and mesenchyme. It participates in blood vessel maturation, emphasizing its significance in vascular development, with a potential role in early heart development. ANGPT1/Angiopoietin-1, Rat (P. pastoris, His) is the recombinant rat-derived ANGPT1/Angiopoietin-1, Rat, expressed by P. pastoris , with N-6*His labeled tag.

ANGPT1, also known as Angiopoietin-1 in rats, functions by binding to and activating the TIE2 receptor, inducing its tyrosine phosphorylation. This protein is implicated in endothelial developmental processes, distinct from the action of VEGF, and appears to play a crucial role in mediating reciprocal interactions between the endothelium and the surrounding matrix and mesenchyme. Additionally, ANGPT1 is involved in mediating blood vessel maturation and stability, suggesting its significance in vascular development. Notably, it may play an important role in the early development of the heart.

Rat

P. pastoris

N-6*His

O35460 (S20-F497)

89807  [NCBI]

Full Length of Mature Protein

SNQRRSPENGGRRYNRIQHGQCAYTFILPEHDGNCRESATEQYNTNALQRDAPHVETDFSSQKLQHLEHVMENYTQWLQKLENYIVENMKSEMAQIQQNAVQNHTATMLEIGTSLLSQTAEQTRKLTDVETQVLNQTSRLEIQLLENSLSTYELEKQLLQQTNEILKIQEKNSLLEHKILEMEGKHKEELDTLKEEKENLQGLVTRQTFIIQELEKQLSRATSNNSVLQKQQLELMDTVHNLVSLCTKEVLLKGGKREEEKPFRDCADVYQAGFNKSGIYTIYFNNMPEPKKVFCNMDVNEGGWTVIQHREDGSLDFQRGWKEYKMGFGNPSGEYWLGNEFIFAITSQRQYMLRIELMDWEGNRAYSQYDRFHIGNQKQNYRLYLKGHTGTAGKQSSLILHGADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNHGKLNGIKWHYFKGPSYSLRSTTMMIRPLDF

57 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4 or 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.