1. Recombinant Proteins
  2. Cytokines and Growth Factors CD Antigens Animal-free Recombinant Proteins
  3. TNF Superfamily T Cell CD Proteins NK Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins
  4. TNF Superfamily Ligands CD178/FasL
  5. Fas Ligand (FasL)
  6. Animal-Free Fas Ligand Protein, Mouse (His)

Animal-Free Fas Ligand Protein, Mouse (His)

Cat. No.: HY-P700176AF
COA Handling Instructions

The cytoplasmic form of Fas ligand protein exerts powerful effects by inhibiting gene transcription. This protein plays a crucial role in regulating cellular processes by inhibiting the expression of specific genes. Animal-Free Fas Ligand Protein, Mouse (His) is the recombinant mouse-derived animal-FreeFas Ligand protein, expressed by E. coli , with N-His labeled tag. The total length of Animal-Free Fas Ligand Protein, Mouse (His) is 152 a.a., with molecular weight of ~18.14 kDa.

Animal-free recombinant proteins offer high consistency and stability, whithout using or contacting of any animal-derived materials and reagents.

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
2 μg $55 In-stock
10 μg $150 In-stock
50 μg $420 In-stock
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The cytoplasmic form of Fas ligand protein exerts powerful effects by inhibiting gene transcription. This protein plays a crucial role in regulating cellular processes by inhibiting the expression of specific genes. Animal-Free Fas Ligand Protein, Mouse (His) is the recombinant mouse-derived animal-FreeFas Ligand protein, expressed by E. coli , with N-His labeled tag. The total length of Animal-Free Fas Ligand Protein, Mouse (His) is 152 a.a., with molecular weight of ~18.14 kDa.

Background

Fas Ligand Protein, in its cytoplasmic form, exerts a powerful impact by inhibiting gene transcription. This protein plays a crucial role in regulating cellular processes by suppressing the expression of specific genes. By preventing gene transcription, Fas Ligand Protein can modulate various biological pathways and influence cellular behavior. Understanding the mechanisms by which this protein functions can provide insights into gene regulation and potentially lead to the development of innovative approaches for controlling gene expression in various biological systems.

Biological Activity

Measure by its ability to induce apoptosis in Jurkat cells. The ED50 for this effect is <1 μg /mL.

Species

Mouse

Source

E. coli

Tag

N-His

Accession

Q544E9 (Q128-L279)

Gene ID

14103  [NCBI]

Molecular Construction
N-term
His
Fas Ligand (Q128-L279)
Accession # Q544E9
C-term
Synonyms
soluble Fas Ligand (sFasL); TNFSF6; CD95L; Apo I Ligand; APTL; APT1LG1; CD178; Fas-Lg; Tnfs; Tnlg1a; gld
AA Sequence

QIANPSTPSEKKEPRSVAHLTGNPHSRSIPLEWEDTYGTALISGVKYKKGGLVINETGLYFVYSKVYFRGQSCNNQPLNHKVYMRNSKYPEDLVLMEEKRLNYCTTGQIWAHSSYLGAVFNLTSADHLYVNISQLSLINFEESKTFFGLYKL

Molecular Weight

Approximately 18.14 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a solution containing 1X PBS, pH 8.0.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Animal-Free Fas Ligand Protein, Mouse (His)
Cat. No.:
HY-P700176AF
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