Animal-Free IL-1F10/IL-38 Protein, Human (His)

IL-1F10/IL-38 Protein is a member of the interleukin 1 cytokine family that regulates adapted and innate immune responses^[1]. Animal-Free IL-1F10/IL-38 Protein, Human (His) is the recombinant human-derived animal-FreeIL-1F10/IL-38 protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.

IL-1F10/IL-38 Protein is a secreted cytokine belonging to the IL-1 family that has immunomodulatory activity. IL-1F10/IL-38 Protein alone does not induce cytokine production, but reduces IL22 and IL17A production by T-cells in response to heat-killed Candida albicans. IL-1F10/IL-38 Protein reduces IL36G-induced production of IL8 by peripheral blood mononuclear cells. IL-1F10/IL-38 Protein increases IL6 production by dendritic cells stimulated by bacterial lipopolysaccharides (LPS). Ligand for IL-36R/IL1RL2^[3][4].

Measured by its ability to inhibit IL-6 secretion by THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 278.5 ng/mL, corresponding to a specific activity is 3590.664 U/mg.

• 
  Measured by its ability to inhibit IL-6 secretion by THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 278.5 ng/mL, corresponding to a specific activity is 3590.664 U/mg.

Human

E. coli

C-6*His

AAI03967.1 (M1-W152)

84639  [NCBI]

Full Length

MCSLPMARYYIIKYADQKALYTRDGQLLVGDPVADNCCAEKICTLPNRGLDRTKVPIFLGIQGGSRCLACVETEEGPSLQLEDVNIEELYKGGEEATRFTFFQSSSGSAFRLEAAAWPGWFLCGPAEPQQPVQLTKESEPSARTKFYFEQSW

Approximately 21 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is recommended to reconstitute to a concentration of 100-200 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Pooria Fazeli, et al. An overview of the biological and multifunctional roles of IL-38 in different infectious diseases and COVID-19. Immunol Res. 2022; 70(3): 316–324.

  [2]. John E Sims, et al. The IL-1 family: regulators of immunity. Nat Rev Immunol. 2010 Feb;10(2):89-102.  [Content Brief]

  [3]. Frank L van de Veerdonk, et al. IL-38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist. Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):3001-5.  [Content Brief]

  [4]. Alejandro Diaz-Barreiro, et al. Multifaceted roles of IL-38 in inflammation and cancer. Cytokine. 2022 Mar:151:155808.  [Content Brief]