1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Protease Inhibitors
  4. Serpin (Protease Inhibitor)
  5. Antithrombin III
  6. Antithrombin III/Serpin C1 Protein, Cynomolgus (HEK293, His)

Antithrombin III/Serpin C1 Protein, Cynomolgus (HEK293, His)

Cat. No.: HY-P75581
COA Handling Instructions

Antithrombin III, also known as Serpin C1, forms a protease inhibitory heterodimer with TMPRSS7. This interaction exemplifies the regulatory role of antithrombin III as a serine protease inhibitor, highlighting its ability to modulate TMPRSS7 activity. Antithrombin III/Serpin C1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Antithrombin III/Serpin C1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Antithrombin III/Serpin C1 Protein, Cynomolgus (HEK293, His) is 430 a.a., with molecular weight of ~55-75 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

Antithrombin III, also known as Serpin C1, forms a protease inhibitory heterodimer with TMPRSS7. This interaction exemplifies the regulatory role of antithrombin III as a serine protease inhibitor, highlighting its ability to modulate TMPRSS7 activity. Antithrombin III/Serpin C1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Antithrombin III/Serpin C1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Antithrombin III/Serpin C1 Protein, Cynomolgus (HEK293, His) is 430 a.a., with molecular weight of ~55-75 kDa.

Background

Antithrombin III (ATIII), also known as Serpin C1, is a crucial serine protease inhibitor that forms a protease inhibiting heterodimer with TMPRSS7. This interaction underlines ATIII's role in regulating coagulation processes by inhibiting the activity of various proteases involved in the blood clotting cascade. The formation of a heterodimer with TMPRSS7 emphasizes the specificity of ATIII in targeting and modulating the activity of specific proteases. This protease inhibiting function plays a central role in preventing excessive blood clotting and maintaining hemostatic balance.

Biological Activity

Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is 2.185 nM, as measured under the described conditions.

Species

Cynomolgus

Source

HEK293

Tag

C-His

Accession

A0A2K5UYN7 (S35-S464)

Gene ID

102117641  [NCBI]

Molecular Construction
N-term
AT-III (S35-S464)
Accession # A0A2K5UYN7
His
C-term
Synonyms
Antithrombin-III; ATIII; Serpin C1; SERPINC1; AT3
AA Sequence

SPVDICTAKPRDIPMNPMCIYRSPEKKATEDEGSEQKIPEATNRRVWELSKANSRFATTFYQHLADSKNDKDNIFLSPLSVSTAFAMTKLGACNDTLKQLMEVFKFDTISEKTSDQIHFFFAKLNCRLYRKANKSSKLVSANRLFGDKSLTFNETYQDISELVYGAKLQPLDFKENAEQSRAAINKWVSNKTEGRITDVIPPEAINELTVLVLVNTIYFKGLWKSKFSPENTRMEPFYKADGESCSASMMYQEGKFRYRRVAEGTQVLELPFKGDDITMVLILPKPEKSLTKVEQELTPEVLQEWLDELEEMMLVVHMPRFRIEDGFSLKEQLQDMGLVDLFSPEKSKLPGIVAEGRDDLYVSDAFHKAFLEVNEEGSEAAASTAIGIAGRSLNPNRVTFKANRPFLVFIREVPLNTIIFMGRVANPCVS

Molecular Weight

Approximately 55-75 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Antithrombin III/Serpin C1 Protein, Cynomolgus (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Antithrombin III/Serpin C1 Protein, Cynomolgus (HEK293, His)
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HY-P75581
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