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  4. B7-H2/ICOSLG B7-H2/CD275
  5. B7-H2/ICOSLG Protein, Cynomolgus (HEK293, Fc)

B7-H2/ICOSLG Protein, Cynomolgus (HEK293, Fc)

Cat. No.: HY-P76394
Handling Instructions

B7-H2 is the ligand of Inducible Costimulator, was a ligand for CD28 and CTLA-4 in human, whereas these interactions were not conserved in mouse. B7-H2 interacted with CD28 in a non-overlapping domain. B7-H2 costimulation via CD28 induced survival factor Bcl-xL, downregulated cell cycle inhibitor p27kip1 and triggered signaling cascade of ERK and AKT kinases-dependent pathways. B7-H2/ICOSLG Protein, Cynomolgus (HEK293, Fc) is the recombinant cynomolgus-derived B7-H2/ICOSLG protein, expressed by HEK293 , with C-hFc labeled tag. The total length of B7-H2/ICOSLG Protein, Cynomolgus (HEK293, Fc) is 256 a.a., with molecular weight of ~53.3 KDa.

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Description

B7-H2 is the ligand of Inducible Costimulator, was a ligand for CD28 and CTLA-4 in human, whereas these interactions were not conserved in mouse. B7-H2 interacted with CD28 in a non-overlapping domain. B7-H2 costimulation via CD28 induced survival factor Bcl-xL, downregulated cell cycle inhibitor p27kip1 and triggered signaling cascade of ERK and AKT kinases-dependent pathways[1][2]. B7-H2/ICOSLG Protein, Cynomolgus (HEK293, Fc) is the recombinant cynomolgus-derived B7-H2/ICOSLG protein, expressed by HEK293 , with C-hFc labeled tag. The total length of B7-H2/ICOSLG Protein, Cynomolgus (HEK293, Fc) is 256 a.a., with molecular weight of ~53.3 KDa.

Background

B7-H2 interacted with CD28 in a non-overlapping domain as B7-1 and B7-2, and was essential for the costimulation of human T cells’ primary responses to allogeneic antigens and memory recall responses. B7-H2 is a costimulatory ligand for CD28. The non-competing nature of B7-H2 and B7-1or B7-2 for binding CD28 suggests these ligands could work independently, simultaneously, and even synergistically to provide maximal CD28 costimulation for T cells[1].

Species

Cynomolgus

Source

HEK293

Tag

C-hFc

Accession

XP_005548618 (M1-T256)

Gene ID

102132485  [NCBI]

Molecular Construction
N-term
B7-H2 (M1-T256)
Accession # XP_005548618
hFc
C-term
Synonyms
ICOS ligand; B7-H2; ICOSLG; LICOS; B7RP-1; CD275
Molecular Weight

Approximately 53.3 kDa.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
B7-H2/ICOSLG Protein, Cynomolgus (HEK293, Fc)
Cat. No.:
HY-P76394
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