BACE1 Protein, Human (HEK293, Fc)

BACE1 protein is crucial in proteolytic processing of amyloid precursor protein (APP), initiating cleavage at the N-terminus of the A-beta peptide sequence.This process results in the extracellular release of beta-cleaved soluble APP and a cell-associated C-terminal fragment.BACE1's cleavage activity extends to CHL1, demonstrating its versatility in substrate processing.BACE1 Protein, Human (HEK293, Fc) is the recombinant human-derived BACE1 protein, expressed by HEK293 , with C-hFc labeled tag.

The BACE1 protein plays a pivotal role in the proteolytic processing of the amyloid precursor protein (APP). It initiates cleavage at the N-terminus of the A-beta peptide sequence, specifically between residues 671 and 672 of APP, resulting in the production and subsequent extracellular release of beta-cleaved soluble APP. Simultaneously, BACE1 generates a corresponding cell-associated C-terminal fragment, subsequently released by gamma-secretase. In addition to its primary target, BACE1 also exhibits cleavage activity on CHL1, as observed through its similarity in cleaving this substrate.

Human

HEK293

C-hFc

P56817-1 (T22-T457)

23621  [NCBI]

Extracellular Domain

TQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMT

Approximately 100-105 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

≥ 95%, as determined by Bis-Tris PAGE.

<1 EU/μg, determined by LAL method.