1. Recombinant Proteins
  2. Cytokines and Growth Factors CD Antigens Biotinylated Proteins
  3. TNF Superfamily B Cell CD Proteins Macrophage CD Proteins
  4. TNF Superfamily Ligands CD257/BAFF
  5. B-cell Activating Factor (BAFF)
  6. BAFF/TNFSF13B Protein, Cynomolgus (Biotinylated, HEK293, His-Flag-Avi)

BAFF/TNFSF13B Protein, Cynomolgus (Biotinylated, HEK293, His-Flag-Avi)

Cat. No.: HY-P77572
COA Handling Instructions

BAFF/TNFSF13B protein is a member of the TNF family and regulates immune responses and cell survival. It is also known as B cell activating factor, which plays a vital role in the development, activation and survival of B cells by binding to receptors such as BAFF-R. BAFF/TNFSF13B Protein, Cynomolgus (Biotinylated, HEK293, His-Flag-Avi) is the recombinant cynomolgus-derived BAFF/TNFSF13B protein, expressed by HEK293 , with N-His, N-Avi, N-Flag labeled tag. The total length of BAFF/TNFSF13B Protein, Cynomolgus (Biotinylated, HEK293, His-Flag-Avi) is 145 a.a., with molecular weight of 55-60 kDa.

For research use only. We do not sell to patients.

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Description

BAFF/TNFSF13B protein is a member of the TNF family and regulates immune responses and cell survival. It is also known as B cell activating factor, which plays a vital role in the development, activation and survival of B cells by binding to receptors such as BAFF-R. BAFF/TNFSF13B Protein, Cynomolgus (Biotinylated, HEK293, His-Flag-Avi) is the recombinant cynomolgus-derived BAFF/TNFSF13B protein, expressed by HEK293 , with N-His, N-Avi, N-Flag labeled tag. The total length of BAFF/TNFSF13B Protein, Cynomolgus (Biotinylated, HEK293, His-Flag-Avi) is 145 a.a., with molecular weight of 55-60 kDa.

Background

The BAFF/TNFSF13B protein belongs to the tumor necrosis factor (TNF) family, a group of cytokines involved in regulating immune responses and cell survival. BAFF/TNFSF13B, also known as B-cell activating factor, plays a crucial role in B-cell development, activation, and survival. It binds to its receptors, including BAFF receptor (BAFF-R), and promotes B-cell proliferation, antibody production, and the maintenance of mature B cells. BAFF/TNFSF13B is particularly important in the development and function of the immune system, as it helps to regulate B-cell homeostasis and immune tolerance. Dysregulation of BAFF/TNFSF13B signaling has been implicated in various autoimmune diseases and B-cell malignancies. Understanding the role of BAFF/TNFSF13B in normal physiology and disease pathology can provide valuable insights into potential therapeutic approaches for conditions involving B-cell dysfunction or dysregulation.

Biological Activity

Immobilized Biotinylated Cynomolgus BAFF (Trimer) , His Tag at 5 μg/mL (100 μl/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for Human BAFFR, hFc Tag with the EC50 of ≤ 0.12 μg/mL determined by ELISA.

Species

Cynomolgus

Source

HEK293

Tag

N-His;N-Avi;N-Flag

Accession

A0A2K5V2X4 (T141-L285)

Gene ID

102123214  [NCBI]

Molecular Construction
N-term
His-Avi-Flag
BAFF (T141-L285)
Accession # A0A2K5V2X4
C-term
Synonyms
BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Molecular Weight

50-60 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
BAFF/TNFSF13B Protein, Cynomolgus (Biotinylated, HEK293, His-Flag-Avi)
Cat. No.:
HY-P77572
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