BRSK1 Protein, Human (Sf9, GST)

The BRSK1 protein is a critical serine/threonine protein kinase that regulates cortical neuronal polarization and centrosome duplication. BRSK1 is phosphorylated and activated by STK11/LKB1, affecting neuronal polarization through MAPT/TAU phosphorylation (“Thr-529” and “Ser-579”) and WEE1 phosphorylation (“Ser-642”). BRSK1 Protein, Human (Sf9, GST) is the recombinant human-derived BRSK1 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of BRSK1 Protein, Human (Sf9, GST) is 777 a.a., .

BRSK1 Protein functions as a pivotal serine/threonine-protein kinase crucial for the polarization of neurons and centrosome duplication. Upon phosphorylation and activation by STK11/LKB1, BRSK1 assumes a central role in regulating the polarization of cortical neurons, likely through the phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579.' Additionally, it participates in neuron polarization by mediating the phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to the down-regulation of WEE1 activity in polarized neurons. In neurons, BRSK1 localizes to synaptic vesicles and contributes to neurotransmitter release, possibly through the phosphorylation of RIMS1. Furthermore, it acts as a positive regulator of centrosome duplication by phosphorylating gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131,' facilitating the translocation of gamma-tubulin and its associated proteins to the centrosome. BRSK1 also participates in the UV-induced DNA damage checkpoint response, potentially inhibiting CDK1 activity through the phosphorylation and activation of WEE1, along with the inhibition of CDC25B and CDC25C.

Human

Sf9 insect cells

N-GST

Q8TDC3 (S2-P778)

84446

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.