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  4. Butyrylcholinesterase/BCHE Protein, Mouse (HEK293, His)

Butyrylcholinesterase/BCHE Protein, Mouse (HEK293, His)

Cat. No.: HY-P72859
COA Handling Instructions

Butyrylcholinesterase (BCHE) Protein, an esterase with broad substrate specificity, crucially inactivates acetylcholine, maintaining neurotransmitter homeostasis. Beyond acetylcholine metabolism, BCHE degrades neurotoxic organophosphates, highlighting its detoxification role. The enzyme's versatility underscores its critical function in safeguarding against harmful effects of neurotoxic compounds. Butyrylcholinesterase/BCHE Protein, Mouse (HEK293, His) is the recombinant mouse-derived Butyrylcholinesterase/BCHE protein, expressed by HEK293 , with C-His labeled tag. The total length of Butyrylcholinesterase/BCHE Protein, Mouse (HEK293, His) is 576 a.a., with molecular weight of 60-70 kDa.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Butyrylcholinesterase (BCHE) Protein, an esterase with broad substrate specificity, crucially inactivates acetylcholine, maintaining neurotransmitter homeostasis. Beyond acetylcholine metabolism, BCHE degrades neurotoxic organophosphates, highlighting its detoxification role. The enzyme's versatility underscores its critical function in safeguarding against harmful effects of neurotoxic compounds. Butyrylcholinesterase/BCHE Protein, Mouse (HEK293, His) is the recombinant mouse-derived Butyrylcholinesterase/BCHE protein, expressed by HEK293 , with C-His labeled tag. The total length of Butyrylcholinesterase/BCHE Protein, Mouse (HEK293, His) is 576 a.a., with molecular weight of 60-70 kDa.

Background

Butyrylcholinesterase (BCHE) protein, functioning as an esterase, exhibits a broad substrate specificity, playing a pivotal role in the inactivation of the neurotransmitter acetylcholine. Beyond its involvement in acetylcholine metabolism, this enzyme demonstrates the capacity to degrade neurotoxic organophosphate esters, showcasing its significance in detoxification processes. The versatility of BCHE underscores its critical function in maintaining neurotransmitter homeostasis and safeguarding against the harmful effects of certain neurotoxic compounds.

Biological Activity

Measured by its ability to cleave Butyrylthiocholine and the specific activity is > 70 nmol/min/μg.

Species

Mouse

Source

HEK293

Tag

C-His

Accession

Q03311 (H28-L603)

Gene ID
Molecular Construction
N-term
BCHE (H28-L603)
Accession # Q03311
His
C-term
Synonyms
Cholinesterase; Acylcholine acylhydrolase; Choline esterase II; BCHE
AA Sequence

HTEEDFIITTKTGRVRGLSMPVLGGTVTAFLGIPYAQPPLGSLRFKKPQPLNKWPDIHNATQYANSCYQNIDQAFPGFQGSEMWNPNTNLSEDCLYLNVWIPVPKPKNATVMVWIYGGGFQTGTSSLPVYDGKFLARVERVIVVSMNYRVGALGFLAFPGNPDAPGNMGLFDQQLALQWVQRNIAAFGGNPKSITIFGESAGAASVSLHLLCPQSYPLFTRAILESGSSNAPWAVKHPEEARNRTLTLAKFTGCSKENEMEMIKCLRSKDPQEILRNERFVLPSDSILSINFGPTVDGDFLTDMPHTLLQLGKVKKAQILVGVNKDEGTAFLVYGAPGFSKDNDSLITRKEFQEGLNMYFPGVSRLGKEAVLFYYVDWLGEQSPEVYRDALDDVIGDYNIICPALEFTKKFAELENNAFFYFFEHRSSKLPWPEWMGVMHGYEIEFVFGLPLGRRVNYTRAEEIFSRSIMKTWANFAKYGHPNGTQGNSTMWPVFTSTEQKYLTLNTEKSKIYSKLRAPQCQFWRLFFPKVLEMTGDIDETEQEWKAGFHRWSNYMMDWQNQFNDYTSKKESCTAL

Molecular Weight

60-70 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 25 mM Tris, 100 mM NaCl, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Butyrylcholinesterase/BCHE Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Butyrylcholinesterase/BCHE Protein, Mouse (HEK293, His)
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HY-P72859
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