Cadherin-1/CD324 Protein, Human (HEK293, Fc)

The Cadherin-1/CD324 protein is a calcium-dependent cell adhesion protein that interacts with homology in junctional cells to facilitate cell type sorting. CDH1 is involved in regulating intercellular adhesion, mobility, and epithelial cell proliferation. Cadherin-1/CD324 Protein, Human (HEK293, Fc) is the recombinant human-derived Cadherin-1/CD324 protein, expressed by HEK293 , with C-hFc labeled tag.

Cadherin-1 (CD324) is a calcium-dependent cell adhesion protein known for its preference to interact homophilically with itself in adjacent cells, thereby contributing to the sorting of heterogeneous cell types. CDH1, a member of the cadherin family, plays a crucial role in regulating cell-cell adhesions, influencing the mobility and proliferation of epithelial cells. Notably, CDH1 exhibits a potent role as an invasive suppressor and serves as a ligand for integrin alpha-E/beta-7E, impacting non-amyloidogenic degradation of Abeta precursors. Moreover, CDH1 demonstrates a robust inhibitory effect on the production of APP C99 and C83, further emphasizing its multifaceted involvement in cellular processes and its potential implications in pathological contexts.

Measured by the ability of the immobilized protein to support the adhesion of MCF-7 cells. The ED₅₀ for this effect is 0.6742 μg/mL, corresponding to a specific activity is 1.483×10³ U/mg.

• 
  Measured by the ability of the immobilized protein to support the adhesion of MCF-7 cells. The ED₅₀ for this effect is 0.6742 μg/mL, corresponding to a specific activity is 1.483×10³ U/mg.

Human

HEK293

C-hFc

P12830-1 (Q23-I707)

999  [NCBI]

Extracellular Domain

QEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFEDCTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNTVGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQI

105-126 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.