CaMKII alpha/CAMK2A Protein, Human (sf9, GST)

CaMKII α/CAMK2A protein functions autonomously upon Ca(2+)/calmodulin binding and autophosphorylation. CaMKII alpha/CAMK2A Protein, Human (sf9, GST) is the recombinant human-derived CaMKII alpha/CAMK2A protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of CaMKII alpha/CAMK2A Protein, Human (sf9, GST) is 478 a.a., with molecular weight of ~80 kDa.

CaMKII alpha/CAMK2A protein, a calcium/calmodulin-dependent kinase, functions autonomously following Ca(2+)/calmodulin-binding and autophosphorylation, playing a crucial role in various cellular processes such as synaptic plasticity, neurotransmitter release, and long-term potentiation. As a member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR, thereby influencing excitatory synaptic transmission. CaMKII alpha also contributes to dendritic spine development and regulates the migration of developing neurons. Beyond these functions, it phosphorylates the transcription factor FOXO3 to activate its transcriptional activity and targets the transcription factor ETS1 in response to calcium signaling, decreasing ETS1 affinity for DNA. In response to interferon-gamma (IFN-gamma) stimulation, it catalyzes the phosphorylation of STAT1, thereby stimulating the JAK-STAT signaling pathway. Additionally, in response to interferon-beta (IFN-beta) stimulation, CaMKII alpha stimulates the JAK-STAT signaling pathway and acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity.

The specific activity was determined to be 160 nmol/min/mg using Autocamtide-2 synthetic peptide (KKALRRQETVDAL-amide) as substrate.

Human

Sf9 insect cells

N-GST

Q9UQM7-1 (M1-H478)

815  [NCBI]

Approximately 80 kDa

Greater than 85% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM Reduced Glutathione, pH 8.0

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.