Carboxypeptidase B1/CPB1 Protein, Human (HEK293, His)

Carboxypeptidase B1/CPB1 Protein, Human (HEK293, His) is an approximately 47.0 kDa carboxypeptidase B1 protein with a C-terminal His-flag.

Carboxypeptidase B1 is encoded by the CPB1 gene. The deduced amino acid sequence of human CPB1 consists of a signal peptide (residues 1 to 15), a pro region (residue 16 to 110), and a mature chain (residues 111 to 417)^[1].
Carboxypeptidase B (CPB) is a protease that specifically hydrolyzes C-terminal alkaline amino acid of a peptide^[1]. As a result, it plays an important role in biological analysis. Carboxypeptidase B has a critical role in downregulating inflammatory responses in rheumatoid arthritis^[2].

Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg. The specific activity is >10000 pmol/min/µg. (Activation description: The proenzyme needs to be activated by Trypsin for an activated form.)

Human

HEK293

C-6*His

P15086/NP_001862.2 (H16-Y417)

1360  [NCBI]

Full Length of Mature Protein

HHGGEHFEGEKVFRVNVEDENHINIIRELASTTQIDFWKPDSVTQIKPHSTVDFRVKAEDTVTVENVLKQNELQYKVLISNLRNVVEAQFDSRVRATGHSYEKYNKWETIEAWTQQVATENPALISRSVIGTTFEGRAIYLLKVGKAGQNKPAIFMDCGFHAREWISPAFCQWFVREAVRTYGREIQVTELLDKLDFYVLPVLNIDGYIYTWTKSRFWRKTRSTHTGSSCIGTDPNRNFDAGWCEIGASRNPCDETYCGPAAESEKETKALADFIRNKLSSIKAYLTIHSYSQMMIYPYSYAYKLGENNAELNALAKATVKELASLHGTKYTYGPGATTIYPAAGGSDDWAYDQGIRYSFTFELRDTGRYGFLLPESQIRATCEETFLAIKYVASYVLEHLY

Approximately 47.0 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filter solution of 20 mM MES, 150 mM NaCl, pH 5.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. K K Yamamoto, et al. Isolation of a cDNA encoding a human serum marker for acute pancreatitis. Identification of pancreas-specific protein as pancreatic procarboxypeptidase B. J Biol Chem. 1992 Feb 5;267(4):2575-81.  [Content Brief]

  [2]. J W Brodrick, et al. Human carboxypeptidase B. II. Purification of the enzyme from pancreatic tissue and comparison with the enzymes present in pancreatic secretion. Biochim Biophys Acta. 1976 Dec 8;452(2):468-81.  [Content Brief]