1. Recombinant Proteins
  2. CD Antigens Receptor Proteins
  3. Erythrocyte CD Proteins Dendritic Cell CD Proteins Pattern Recognition Receptors
  4. DC-SIGN/CD299 C-type Lectin Receptors
  5. DC-SIGN
  6. CD299 Protein, Human (HEK293)

CD299 Protein, Human (HEK293)

Cat. No.: HY-P76218
COA Handling Instructions

CD299 protein showcases an unconventional genetic architecture with a non-canonical intron-exon splice junction, highlighting unique structural features. CD299 Protein, Human (HEK293) is the recombinant human-derived CD299 protein, expressed by HEK293 , with tag free. The total length of CD299 Protein, Human (HEK293) is 322 a.a., with molecular weight of ~37 KDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

CD299 protein showcases an unconventional genetic architecture with a non-canonical intron-exon splice junction, highlighting unique structural features. CD299 Protein, Human (HEK293) is the recombinant human-derived CD299 protein, expressed by HEK293 , with tag free. The total length of CD299 Protein, Human (HEK293) is 322 a.a., with molecular weight of ~37 KDa.

Background

CD299 protein exhibits a non-canonical intron-exon splice junction, emphasizing an unconventional configuration in its genetic architecture.

Species

Human

Source

HEK293

Tag

Tag Free

Accession

Q9H2X3 (Q71-E399)

Gene ID
Molecular Construction
N-term
CD299 (S78-E399)
Accession # Q9H2X3
C-term
Synonyms
C-type lectin domain family 4 member M; DC-SIGNR; DC-SIGN2; L-SIGN; CD299; CLEC4M; CD209L
Molecular Weight

38-50 kDa.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CD299 Protein, Human (HEK293)
Cat. No.:
HY-P76218
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