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  4. Ebola Viral Protein 24
  5. Ebola virus VP24 Protein (AHX24653, His)

Ebola virus VP24 Protein (AHX24653, His)

Cat. No.: HY-P76310
Handling Instructions

Ebola virus VP24 Protein is an IFN antagonist, and mediates nucleocapsid assembly. VP24 inhibits IFN-activated signaling by preventing nuclear import of STAT1 via competitive binding to nuclear import receptors (karyopherins). VP24 can interact with NP and is essential for nucleocapsid formation and packaging into the virion. Ebola virus VP24 Protein (AHX24653, His) is the recombinant Virus-derived Ebola virus VP24 protein, expressed by E. coli , with N-His labeled tag. The total length of Ebola virus VP24 Protein (AHX24653, His) is 233 a.a., with molecular weight of ~28.5 KDa.

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Description

Ebola virus VP24 Protein is an IFN antagonist, and mediates nucleocapsid assembly. VP24 inhibits IFN-activated signaling by preventing nuclear import of STAT1 via competitive binding to nuclear import receptors (karyopherins). VP24 can interact with NP and is essential for nucleocapsid formation and packaging into the virion[1][2][3]. Ebola virus VP24 Protein (AHX24653, His) is the recombinant Virus-derived Ebola virus VP24 protein, expressed by E. coli , with N-His labeled tag. The total length of Ebola virus VP24 Protein (AHX24653, His) is 233 a.a., with molecular weight of ~28.5 KDa.

Background

Ebola virus (EBOV) possesses a single-stranded, negative sense RNA genome of approximately 19 kilobases that encodes seven structural proteins: the nucleoprotein (NP), VP35, VP40, the glycoprotein (GP), VP30, VP24, and the RNA-dependent RNA polymerase L[1]. VP24 is one of two proteins of the ebolaviruses that can antagonize IFN responses (the other is VP35). VP24 inhibits signaling downstream of IFNα/β and IFNγ, by trapping karyopherin α proteins (α1, α5 and α6) in the cytoplasm, and prevents them from shuttling otherwise activated, phosphorylated STAT1 to the nucleus. VP24 also prevents phosphorylation of p38 MAPK[2][3]. Besides, the interaction between NP and VP24 is essential for nucleocapsid formation and packaging into the virion[1]. VP24 is a critical target in the development of anti-EBOV agents.

Species

Virus

Source

E. coli

Tag

N-His

Accession

AHX24653 (M1-G233)

Gene ID

/

Molecular Construction
N-term
His
EBOV VP24 (M1-G233)
Accession # AHX24653
C-term
Synonyms
Ebola virus EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) VP24 Protein; VP24 Protein; EBOV
Molecular Weight

Approximately 28.5 kDa.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Ebola virus VP24 Protein (AHX24653, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Ebola virus VP24 Protein (AHX24653, His)
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HY-P76310
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