1. Recombinant Proteins
  2. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators
  3. Ephrin/Eph Family Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. Eph Receptors
  5. EphB3
  6. EphB3 Protein, Mouse (537a.a, HEK293, His)

EphB3 Protein, Mouse (537a.a, HEK293, His)

Cat. No.: HY-P75749
COA Handling Instructions

The EphB3 protein is a receptor tyrosine kinase that binds to the transmembrane ephrin B ligand to initiate bidirectional signaling. The forward signal originates from the receptor, while the reverse signal originates from the ephrin ligand. EphB3 Protein, Mouse (537a.a, HEK293, His) is the recombinant mouse-derived EphB3 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphB3 Protein, Mouse (537a.a, HEK293, His) is 537 a.a., with molecular weight of ~63 kDa.

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Description

The EphB3 protein is a receptor tyrosine kinase that binds to the transmembrane ephrin B ligand to initiate bidirectional signaling. The forward signal originates from the receptor, while the reverse signal originates from the ephrin ligand. EphB3 Protein, Mouse (537a.a, HEK293, His) is the recombinant mouse-derived EphB3 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphB3 Protein, Mouse (537a.a, HEK293, His) is 537 a.a., with molecular weight of ~63 kDa.

Background

The EphB3 protein, a receptor tyrosine kinase, engages in promiscuous binding to transmembrane ephrin-B family ligands on adjacent cells, initiating contact-dependent bidirectional signaling. The downstream pathway originating from the receptor is known as forward signaling, while the signaling pathway downstream of the ephrin ligand is termed reverse signaling. EphB3 generally exhibits an overlapping and redundant function with EPHB2, particularly in axon guidance during development, regulating neurons forming the corpus callosum and the anterior commissure—two major interhemispheric connections between the temporal lobes of the cerebral cortex. Alongside its role in axon guidance, EphB3 plays a crucial redundant role with other ephrin-B receptors in the development and maturation of dendritic spines and the formation of excitatory synapses. The protein also governs various aspects of development, including angiogenesis, palate development, and thymic epithelium development. Through both forward and reverse signaling within the EFNB2/EPHB3 complex, EphB3 regulates the migration and adhesion of cells involved in tubularizing the urethra and septating the cloaca. Notably, EphB3 plays a significant role in intestinal epithelium differentiation by segregating progenitor cells from differentiated cells in the crypt.

Biological Activity

Measured by its binding ability in a functional ELISA . Immobilized mouse EphB3 at 2 μg/mL (100 μl/well) can bind mouse EFNB1 with a linear range of 0.1-12.5 ng/mL.

Species

Mouse

Source

HEK293

Tag

C-His

Accession

P54754 (M1-T537)

Gene ID

13845  [NCBI]

Synonyms
Ephrin type-B receptor 3; mDK-5; Tyrosine-protein kinase receptor SEK-4; Sek4
Molecular Weight

Approximately 63 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
EphB3 Protein, Mouse (537a.a, HEK293, His)
Cat. No.:
HY-P75749
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