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  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Flap endonuclease 1/FEN-1 Protein, Human (His, Myc)

Flap endonuclease 1/FEN-1 Protein, Human (His, Myc)

Cat. No.: HY-P700010
COA Handling Instructions

Flap endonuclease 1 (FEN-1) is a structure-specific nuclease crucial for DNA replication and repair. In replication, it cleaves 5'-overhanging flaps, guiding DNA polymerase and creating a nick for ligation. FEN-1 is integral to the long patch base excision repair pathway, preventing genomic instability. It exhibits 5'-3' exonuclease and RNase H activities, playing roles in rDNA and mitochondrial DNA replication and repair. Flap endonuclease 1/FEN-1 Protein, Human (His, Myc) is the recombinant human-derived Flap endonuclease 1/FEN-1 protein, expressed by E. coli , with N-10*His, C-Myc labeled tag. The total length of Flap endonuclease 1/FEN-1 Protein, Human (His, Myc) is 380 a.a., with molecular weight of ~50.0 kDa.

For research use only. We do not sell to patients.

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Description

Flap endonuclease 1 (FEN-1) is a structure-specific nuclease crucial for DNA replication and repair. In replication, it cleaves 5'-overhanging flaps, guiding DNA polymerase and creating a nick for ligation. FEN-1 is integral to the long patch base excision repair pathway, preventing genomic instability. It exhibits 5'-3' exonuclease and RNase H activities, playing roles in rDNA and mitochondrial DNA replication and repair. Flap endonuclease 1/FEN-1 Protein, Human (His, Myc) is the recombinant human-derived Flap endonuclease 1/FEN-1 protein, expressed by E. coli , with N-10*His, C-Myc labeled tag. The total length of Flap endonuclease 1/FEN-1 Protein, Human (His, Myc) is 380 a.a., with molecular weight of ~50.0 kDa.

Background

Flap endonuclease 1 (FEN-1) is a structure-specific nuclease with dual enzymatic activities, functioning in both DNA replication and repair processes. In DNA replication, FEN-1 plays a crucial role by cleaving the 5'-overhanging flap structure generated during displacement synthesis, allowing DNA polymerase to navigate the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and tracks to cleave the flap base, creating a nick for subsequent ligation. Additionally, FEN-1 is integral to the long patch base excision repair (LP-BER) pathway, where it cleaves within the apurinic/apyrimidinic (AP) site-terminated flap. Acting as a genome stabilization factor, FEN-1 prevents the equilibration of flaps into structures that may lead to duplications or deletions. Furthermore, the protein exhibits 5'-3' exonuclease activity on nicked or gapped double-stranded DNA and possesses RNase H activity. FEN-1's versatile functions extend to replication and repair processes in rDNA and mitochondrial DNA.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

E. coli

Tag

N-10*His;C-Myc

Accession

P39748 (M1-K380)

Gene ID
Molecular Construction
N-term
10*His
FEN-1 (M1-K380)
Accession # P39748
C-term
Synonyms
rHuFlap endonuclease 1/FEN-1; Flap Endonuclease 1; FEN-1; DNase IV; Flap Structure-Specific Endonuclease 1; Maturation Factor 1; MF1; hFEN-1; FEN1; RAD2
AA Sequence

MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK

Molecular Weight

Approximately 50.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-HCl, 1mM EDTA, 6% Trehalose, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Flap endonuclease 1/FEN-1 Protein, Human (His, Myc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Flap endonuclease 1/FEN-1 Protein, Human (His, Myc)
Cat. No.:
HY-P700010
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