FEN1 - flap structure-specific endonuclease 1 Gene

Also Known as MF1; RAD2; FEN-1

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 2237

About FEN1

Cytogenetic location: 11q12.2 Genomic coordinates (GRCh38): 11:61,792,911-61,797,238 (from NCBI)

This gene has 3 transcripts (splice variants), 201 orthologues, 2 paralogues and is associated with 38 phenotypes. Broad expression in lymph node (RPKM 16.0), bone marrow (RPKM 13.0) and 24 other tissues.

Summary

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP Endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 Endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit FLAP processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the FLAP that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]

FEN1 Products (1)

mRNA	Protein	Name
NM_004111.6	NP_004102.1	flap endonuclease 1

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables 5'-3' exonuclease activity	IDA IDA: Inferred from direct assay	7961795	GOA
enables 5'-flap endonuclease activity	IDA IDA: Inferred from direct assay	18995831	GOA
enables 5'-flap endonuclease activity	IMP IMP: Inferred from mutant phenotype	8621570	GOA
enables DNA binding	IMP IMP: Inferred from mutant phenotype	11986308	GOA
enables RNA-DNA hybrid ribonuclease activity	IDA IDA: Inferred from direct assay	7961795	GOA
enables flap endonuclease activity	IDA IDA: Inferred from direct assay	18499658	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	9305916	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in DNA replication, removal of RNA primer	IDA IDA: Inferred from direct assay	18995831	GOA
involved in nucleic acid metabolic process	IDA IDA: Inferred from direct assay	18499658	GOA
involved in positive regulation of sister chromatid cohesion	IMP IMP: Inferred from mutant phenotype	18499658	GOA

Cellular Component GO Annotation	Evidence	References	Source
colocalizes with chromosome, telomeric region	IDA IDA: Inferred from direct assay	24270157	GOA
located in mitochondrion	IDA IDA: Inferred from direct assay	18995831	GOA
located in nucleus	IDA IDA: Inferred from direct assay	7961795	GOA
part of protein-containing complex	IDA IDA: Inferred from direct assay	25378300	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

FEN1 Protein Structure

XPG_N

XPG_I

0
100
200
300
380 a.a.

Protein Preferred Names	Protein Names
flap endonuclease 1	DNase IV

FEN1 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	FEN1	P39748	MUS81	Homo sapiens	Q96NY9	Anti Tag CoIP	22551069
Intra	FEN1	P39748	PCNA	Homo sapiens	P12004	Pull Down	26751069
Intra	FEN1	P39748	PCNA	Homo sapiens	P12004	Anti Tag CoIP	33961781
Intra	FEN1	P39748	PCNA	Homo sapiens	P12004	Y2H Pooling	18692475
Intra	FEN1	P39748	PCNA	Homo sapiens	P12004	Anti Tag CoIP	26496610
Intra	FEN1	P39748	PCNA	Homo sapiens	P12004	Anti Tag CoIP	28514442
Intra	FEN1	P39748	PCNA	Homo sapiens	P12004	SLC	31467278
Intra	FEN1	P39748	PCNA	Homo sapiens	P12004	BiFC	26030842
Intra	FEN1	P39748	PCNA	Homo sapiens	P12004	FACS	26030842
Intra	FEN1	P39748	PCNA	Homo sapiens	P12004	Y2H Array	31515488
Intra	FEN1	P39748	PCNA	Homo sapiens	P12004	BRET	29997244
Intra	FEN1	P39748	PCNA	Homo sapiens	P12004	Complementation	31467278
Intra	FEN1	P39748	PCNA	Homo sapiens	P12004	FRET	14657243
Intra	FEN1	P39748	WRN	Homo sapiens	Q14191	Anti Bait CoIP	14657243
Intra	FEN1	P39748	WRN	Homo sapiens	Q14191	IF	14657243
Intra	FEN1	P39748	WRN	Homo sapiens	Q14191	CoIP	14657243
Intra	FEN1	P39748	WRN	Homo sapiens	Q14191	ELISA	14688284
Intra	FEN1	P39748	BLM	Homo sapiens	P54132	Anti Bait CoIP	14688284

Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant FEN1 Proteins

Cat. No.	Product Name	Accession	Purity
HY-P70348	Flap endonuclease 1/FEN-1 Protein, Human	P39748-1 (M1-K380)	≥ 95%, as determined by reducing SDS-PAGE.
HY-P700010	Flap endonuclease 1/FEN-1 Protein, Human (His, Myc)	P39748-1 (M1-K380)	≥ 95%, as determined by reducing SDS-PAGE.

FEN1 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P80669	FEN1 Antibody (YA765)	WB, IP	Human, Mouse, Rat
HY-P80669A	FEN1 Antibody (YA765)(PBS only)	WB, IP	Human, Mouse, Rat
HY-P85602	FEN1 Antibody (YA5294)	WB, ICC/IF	Human, Mouse, Rat, Monkey, Hamster
HY-P85603	FEN1 Antibody (YA5295)	WB, IP	Human, Mouse, Rat

Related Diseases

Diseases	Alias
Werner Syndrome	Werner'S Syndrome WRN Adult Progeria Ws Adult Premature Ageing Syndrome Adult Premature Aging Syndrome Werners Syndrome
Xeroderma Pigmentosum, Variant Type	Xeroderma Pigmentosum XPV Xeroderma Pigmentosum Variant Type Xeroderma Pigmentosum With Normal Dna Repair Rates Photosensitivity With Defective Dna Synthesis Xp De Sanctis-Cacchione Syndrome Desanctis-Cacchione Syndrome Xeroderma Pigmentosa Xerodermic Idiocy Xeroderma Pigmentosum Variant Xp - [Xeroderma Pigmentosum] Atrophoderma Pigmentosum
Vitelliform Macular Dystrophy	Best Disease Juvenile-Onset Vitelliform Macular Dystrophy Macular Dystrophy, Vitelliform Best Macular Dystrophy Vitelliform Dystrophy
Fanconi Anemia, Complementation Group A	Fanconi Anemia Fanconi Pancytopenia Fanconi Anemia Complementation Group A FANCA Fa Fanconi Panmyelopathy Fanconi'S Anemia Fanconi Anaemia Fanconi'S Anaemia Fanconi Hypoplastic Anemia Estren-Dameshek Variant Of Fanconi Anemia Estren-Dameshek Variant Of Fanconi Pancytopenia Fanconi Anemia Estren-Dameshek Variant Fanconis Anemia
Bloom Syndrome	BLM Bs Bls Bloom-Torre-Machacek Syndrome Microcephaly, Growth Restriction, And Increased Sister Chromatid Exchange 1 Mgrisce1 Congenital Telangiectatic Erythema Congenital Telangiectatic Erythema Syndrome Growth Deficiency, Sun-Sensitive, Telangiectatic, Hypo And Hyperpigmented Skin, Predisposition To Malignancy And Chromosomal Instability Bloom'S Syndrome Bsyn
Xeroderma Pigmentosum, Complementation Group G	Xeroderma Pigmentosum, Group G Xeroderma Pigmentosum Vii Xp7 XPG Xeroderma Pigmentosum Group G Xp Group G Xp, Group G Xpgc Xeroderma Pigmentosum, Group G/Cockayne Syndrome Xeroderma Pigmentosum, Type 7 Xeroderma Pigmentosum Complementation Group G XP-G Xp-G/Cs Xeroderma Pigmentosum Group G/Cockayne Syndrome
Cockayne Syndrome	Cockayne'S Syndrome Dwarfism-Retinal Atrophy-Deafness Syndrome Neill-Dingwall Syndrome Progeria-Like Syndrome Progeroid Nanism Cs
Huntington Disease	Huntington'S Disease Huntington Chorea Huntington'S Chorea HD Huntington Chronic Progressive Hereditary Chorea Juvenile Huntington Disease Chronic Progressive Chorea Chronic Progressive Hereditary Chorea Hc - [Huntington Chorea] Hereditary Chorea Progressive Hereditary Chorea
Ataxia, Early-Onset, With Oculomotor Apraxia And Hypoalbuminemia	Aoa1 Ataxia-Telangiectasia-Like Disorder EAOH Eoca-Ha Ataxia With Oculomotor Apraxia Type 1 Ataxia-Oculomotor Apraxia 1 Ataxia-Oculomotor Apraxia Syndrome AOA Ataxia-Telangiectasia-Like Syndrome Ataxia-Oculomotor Apraxia Type 1 Ataxia With Oculomotor Apraxia Cerebellar Ataxia, Early-Onset, With Hypoalbuminemia Early-Onset Ataxia With Oculomotor Apraxia And Hypoalbuminemia Early-Onset Cerebellar Ataxia With Hypoalbuminemia Adult Onset Ataxia With Oculomotor Apraxia Early-Onset Ataxia With Ocular Motor Apraxia And Hypoalbuminemia Scan2 Scar1 Spinocerebellar Ataxia With Axonal Neuropathy Type 2 Spinocerebellar Ataxia, Recessive, Non-Friedreich Type 1 Atld Ataxia Early-Onset With Oculomotor Apraxia And Hypoalbuminemia Cerebellar Ataxia Early-Onset With Hypoalbuminemia Ataxia-Oculomotor Apraxia Spinocerebellar Ataxia, Autosomal Recessive 1
Rothmund-Thomson Syndrome, Type 2	Rothmund-Thomson Syndrome Rts RTS2 Poikiloderma Of Rothmund-Thomson Rothmund-Thomson Syndrome Type 2 Congenital Poikiloderma Poikiloderma Congenitale Poikiloderma Atrophicans And Cataract Poikiloderma Congenitale Of Rothmund-Thomson Poikiloderma Of Rothmund-Thomson Type 2 Rothmund-Thomson Syndrome 2 Erythrokeratodermia Variabilis
Spinocerebellar Ataxia Type 1 With Axonal Neuropathy	Scan1 Spinocerebellar Ataxia With Axonal Neuropathy Type 1 Autosomal Recessive Spinocerebellar Ataxia With Axonal Neuropathy Scan1, Tdp1-Related Spinocerebellar Ataxia With Axonal Neuropathy Spinocerebellar Ataxia Autosomal Recessive With Axonal Neuropathy Spinocerebellar Ataxia With Axonal Neuropathy Spinocerebellar Ataxia, Autosomal Recessive, With Axonal Neuropathy Ataxia, Spinocerebellar, Autosomal Recessive With Axonal Neuropathy
Baller-Gerold Syndrome	BGS Craniosynostosis With Radial Defects Craniosynostosis-Radial Aplasia Syndrome Craniosynostosis Radial Aplasia Syndrome
Developmental And Epileptic Encephalopathy 75	DEE75 Epileptic Encephalopathy, Early Infantile, 75 Eiee75 Developmental And Epileptic Encephalopathy, 75 Early Infantile Epileptic Encephalopathy 75
Cockayne Syndrome B	Cockayne Syndrome Type 2 Cockayne Syndrome, Type B Cockayne Syndrome Type Ii CSB Cockayne Syndrome 2 Cockayne Syndrome Type B Ckn2 Cockayne Syndrome, Type Ii
Colorectal Cancer	Colon Cancer Colorectal Carcinoma Colon Carcinoma Colorectal Cancer, Susceptibility To Carcinoma Of Colon CRC Colorectal Cancer With Chromosomal Instability, Somatic Colon Cancer, Somatic Colon Cancer, Susceptibility To Colonic Neoplasms Colorectal Neoplasms Colorectal Cancer, Somatic Colon Cancer, Advanced, Somatic Colonic Carcinoma Colorectal Carcinomas Colon Cancers Colorectal Cancers Cancer, Colorectal, Somatic Cancer, Colon Cancer, Colorectal, Susceptibility To Colorectal Neoplasm Colonic Neoplasm Malignant Tumor Of Colon
Trichothiodystrophy	Ttd Amish Brittle Hair Syndrome Bids Syndrome Brittle Hair-Intellectual Impairment-Decreased Fertility-Short Stature Syndrome Ibids Pibids Trichothiodystrophy Syndromes
Sezary'S Disease	Sezary Syndrome Sézary Syndrome Sezary'S Lymphoma Sezary Disease Sezary Erythroderma Sezary Lymphoma
Fuchs' Endothelial Dystrophy	Fuchs Endothelial Corneal Dystrophy Fuchs Endothelial Dystrophy Fuchs Dystrophy Fced Fuchs' Corneal Dystrophy Fuchs' Endothelial Corneal Dystrophy Fuchs Atrophy Fuchs Corneal Dystrophy Endoepithelial Corneal Dystrophy Fecd Late Hereditary Endothelial Dystrophy Corneal Dystrophy, Fuchs Endothelial Dystrophy, Corneal, Fuchs Endothelial Corneal Dystrophy, Fuchs' Endothelial, 1
Aplastic Anemia	Aplastic Anemia, Susceptibility To Anemia Aplastic Idiopathic Aplastic Anemia Secondary Aplastic Anemia Idiopathic Bone Marrow Failure Aplastic Anemia Idiopathic AA Anemia, Aplastic Aplastic Anemia, Idiopathic Erythroid Aplasia Aa - [Aplastic Anaemia] Haematopoietic Aplasia Aleukia Haemorrhagica Anaemia Due To Decreased Red Cell Production Aplasia Bone Marrow Aplastic Bone Marrow Hypoplastic Anaemia Nos Myeloid Bone Marrow Aplasia Pancytopenia Panhaematopenia Hypoproliferative Anaemia Medullary Hypoplasia Red Blood Cells Hypoplastic Anaemia Panmyelophthisis Panhemocytopenia Refractive Hypoproliferative Anaemia Toxic Anaemia Toxic Aplastic Anaemia Aplastic Anaemia Due To Toxic Cause Idiopathic Aplastic Anaemia Nos
Lynch Syndrome	Hereditary Nonpolyposis Colon Cancer Hereditary Nonpolyposis Colorectal Cancer Hereditary Nonpolyposis Colorectal Carcinoma Hereditary Nonpolyposis Colorectal Neoplasms Familial Nonpolyposis Colon Cancer Hnpcc Coca 1 Hereditary Defective Mismatch Repair Syndrome Hereditary Non-Polyposis Colon Cancer Hereditary Non-Polyposis Colon Cancer Syndrome Hereditary Non-Polyposis Colorectal Cancer Hereditary Non-Polyposis Colorectal Cancer Syndrome Hereditary Nonpolyposis Colon Cancer Syndrome Hereditary Nonpolyposis Colorectal Cancer Syndrome Hereditary Nonpolyposis Colorectal Neoplasm Hnpcc - Hereditary Nonpolyposis Colon Cancer Cancer Family Syndrome Familial Nonpolyposis Colorectal Cancer Colon Cancer, Familial Nonpolyposis Colorectal Neoplasms, Hereditary Nonpolyposis Cancer, Colorectal, Nonpolyposis, Hereditary Colorectal Cancer, Hereditary Nonpolyposis, Type 1

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (18)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-123834	FEN1-IN-1		99.85%	Unkown
HY-145758	FEN1-IN-SC13	Inhibitor	98.42%	Unkown
HY-136485	FEN1-IN-4	Inhibitor	99.86%	Unkown
HY-122695	FEN1-IN-2	Inhibitor	99.94%	Unkown
HY-177512	MSC778	Inhibitor	/	Unkown
HY-153792	FEN1-IN-7	Inhibitor	99.93%	Unkown
HY-170845	MU1409	Inhibitor	98.52%	Unkown
HY-153790	FEN1-IN-5	Inhibitor	99.79%	Unkown
HY-136484	FEN1-IN-3	Inhibitor	98.06%	Unkown
HY-153791	FEN1-IN-6	Inhibitor	/	Unkown
HY-139678	SC13		/	Unkown
HY-183488	R9-caPep		/	Unkown
HY-185455	FEN1-IN-9	Inhibitor	/	Unkown
HY-RS04861	FEN1 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-175883	FEN1-IN-8	Inhibitor	/	Unkown
HY-183454	JFD00950	Inhibitor	/	Unkown
HY-RS16635	Fen1 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS23070	Fen1 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Canis familiaris	FEN1	VGNC	VGNC:40817
Mus musculus	FEN1	MGD	MGI:102779
Felis catus	FEN1	VGNC	VGNC:82046
Macaca mulatta	FEN1	VGNC	VGNC:72635
Rattus norvegicus	FEN1	RGD	RGD:621821
Bos taurus	FEN1	VGNC	VGNC:28947
Others	FEN1	NCBI