Art. -Nr.: HY-123834

FEN1-IN-1

Name: FEN1-IN-1
Brand: MedChemExpress
SKU: HY-123834
Price: 28.00 USD
Availability: InStock
Rating: 5 (9 reviews)

9 Cited Publications

Kundenbewertung

Based on 9 publication(s) in Google Scholar

FEN1-IN-1 (compound 1) is a small molecule flap endonuclease 1 (FEN1) inhibitor with antitumor activity. FEN1-IN-1 binds to the active site of FEN1 and partly achieves inhibition by the co-ordination of Mg²⁺ ions. FEN1-IN-1 initiaties a DNA damage response and activates the ATM checkpoint signalling pathway, the phosphorylation of histone H2AX and the ubiquitination of FANCD2 in mammalian cells. FEN1-IN-1 is promising for research of cancers.

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Reinheit : 99.85%
CAS. Nr.: 824983-91-7
Formel: C₁₅H₁₂N₂O₅S
Molecular Weight:332.33

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

FEN1-IN-1 Chemical Structure

CAS. Nr.: 824983-91-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (9)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

FEN1^[1]

Alle ATM/ATR Isoform-spezifische Produkte anzeigen :

In Vitro

FEN1-IN-1 (0-30 μM, 3 days) inhibits 212 cell-lines growth with a mean GI 50 of 15.5 μM^[1].
FEN1-IN-1 (0.2-20 μM, 24 h) induces Hela cells disrupted for MRE11A more sensitive and decreasea the survival of the MRE11A deficient cell-lines due to cytotoxicity^[1].
FEN1-IN-1 (0-10 μM, 24 h) inhibits both FEN1 and EXO1 function and induces a DNA damage response to activate the signalling pathways in SW620 and HCT-116 cells^[1].
FEN1-IN-1 (10 μM, 24 h) leads to replication fork instabilities that, if left unrepaired, can lead to cell death^[1].

Tissue-specific sensitivity to FEN1-IN-1 (GI₅₀: μM)^[1]

FEN1-IN-1	Bladder	Breast	Colorectal	Gastric	Liver	Lung	Pancreatic	Prostate	Gynae	Head & Neck
Cell-lines screened	19	22	21	25	21	37-	24	7	21	15

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

CAS. Nr. 824983-91-7
Appearance Solid
Molecular Weight 332.33
Formel C₁₅H₁₂N₂O₅S
Color White to off-white
SMILES

O=C1N(CC2OC3=CC=CC=C3OC2)C(C=CS4)=C4C(N1O)=O
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (9)

All Cell Imaging/StainingBio/Physico-chemical Assay

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Journal Impact Factor

Chem Eng J

2023 Aug 15, 470, 144407.

FEN1-IN-1 purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2023 Aug 15, 470, 144407.

Confocal fluorescence images of SMMC-7721 cells and HepG2 cells which were pre-treated without FEN1 inhibitor (Row 1); with FEN1-IN-1 (Row 2) or ATA (Row 3) for 4 h.

FEN1-IN-1 purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2023 Aug 15, 470, 144407.

The inhibitory effect evaluation of different chemical substances on FEN1. The inhibition assays were conducted by using positive controls (ATA and FEN1-IN-1) and negative controls (curcumin and buffer). In the presence of inhibitors, the fluorescence was weak which suggested that FEN1-IN-1 and ATA have seriously inhibited the activity of FEN1.

FEN1-IN-1 purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2023 Aug 15, 470, 144407.

Fluorescence signal response to the FEN1 activity in SMMC-7721 cell extracts and the inhibitory effect of ATA and FEN1-IN-1 on the FEN1 activity in the SMMC-7721 cell extracts.
Biosens Bioelectron

Homogeneous electrochemical protein assay based on repeated DNA hairpin self-folding and catalyzed extension. [Abstract]

2026 Feb 1:293:118160. PMID: 41151473
Adv Healthc Mater

Ultrasensitive Detection of FEN1 Activity for Cancer Diagnosis Using a CRISPR/Cas13a-Based Triple Cascade Amplification System. [Abstract]

2025 Mar;14(6):e2404411. PMID: 40029034

FEN1-IN-1 purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Mar;14(6):e2404411. [Abstract]

FEN1-IN-1 showed low fluorescence, while all the negative controls exhibited high fluorescence similar to the blank sample that incubated FEN1 with buffer.

FEN1-IN-1 purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Mar;14(6):e2404411. [Abstract]

The relationship between the fluorescence intensities and the concentrations of FEN1-IN-1. Samples containing 5 pM of FEN1 were incubated with 20 pM of chemicals for 30 min, and then subjected to CLAIM analyses. The fluorescence decreased as the FEN1-IN-1 concentrations climbing, indicating the does-dependent inhibition. By fitting the data, the half-maximal inhibitory concentration (IC₅₀) was determined to be 2.3 pM.
Sens Actuators B Chem

2023 Oct 15, 393, 134265.
ACS Appl Mater Interfaces

Self-Powered FEN1 Biosensor Based on Accelerated CRISPR/Cas Trans-Cleavage around Porous Fe3O4 Nanoparticles. [Abstract]

2024 Oct 16;16(41):55181-55188. PMID: 39361498
Anal Chem

Signal-Amplified Detection of the Tumor Biomarker FEN1 Based on Cleavage-Induced Ligation of a Dumbbell DNA Probe and Rolling Circle Amplification. [Abstract]

2021 Feb 16;93(6):3287-3294. PMID: 33529005
Mol Cancer Ther

2026 Jul 15:10.1158/1535-7163.MCT-26-0182. PMID: 42456172
ChemElectroChem

2023 Mar 16.
University of Alberta

2025.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 250 mg/mL (752.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0091 mL

15.0453 mL

30.0906 mL

5 mM

0.6018 mL

3.0091 mL

6.0181 mL

10 mM

0.3009 mL

1.5045 mL

3.0091 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (6.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (6.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (6.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Ward TA, et al. Small molecule inhibitors uncover synthetic genetic interactions of human flap endonuclease 1 (FEN1) with DNA damage response genes. PLoS One. 2017 Jun 19;12(6):e0179278. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0091 mL	15.0453 mL	30.0906 mL	75.2264 mL
	5 mM	0.6018 mL	3.0091 mL	6.0181 mL	15.0453 mL
	10 mM	0.3009 mL	1.5045 mL	3.0091 mL	7.5226 mL
	15 mM	0.2006 mL	1.0030 mL	2.0060 mL	5.0151 mL
	20 mM	0.1505 mL	0.7523 mL	1.5045 mL	3.7613 mL
	25 mM	0.1204 mL	0.6018 mL	1.2036 mL	3.0091 mL
	30 mM	0.1003 mL	0.5015 mL	1.0030 mL	2.5075 mL
	40 mM	0.0752 mL	0.3761 mL	0.7523 mL	1.8807 mL
	50 mM	0.0602 mL	0.3009 mL	0.6018 mL	1.5045 mL
	60 mM	0.0502 mL	0.2508 mL	0.5015 mL	1.2538 mL
	80 mM	0.0376 mL	0.1881 mL	0.3761 mL	0.9403 mL
	100 mM	0.0301 mL	0.1505 mL	0.3009 mL	0.7523 mL

Bioz

Keywords

FEN1-IN-1

9 Cited Publications

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FEN1-IN-1

Publications Citing Use of MedChemExpress (MCE) FEN1-IN-1

Alle ATM/ATR Isoform-spezifische Produkte anzeigen