Flap endonuclease 1/FEN-1 Protein, Human

Flap endonuclease 1 (FEN-1) is a structure-specific nuclease crucial for DNA replication and repair. In replication, it cleaves 5'-overhanging flaps, guiding DNA polymerase and creating a nick for ligation. FEN-1 is integral to the long patch base excision repair pathway, preventing genomic instability. It exhibits 5'-3' exonuclease and RNase H activities, playing roles in rDNA and mitochondrial DNA replication and repair. Flap endonuclease 1/FEN-1 Protein, Human is the recombinant human-derived Flap endonuclease 1/FEN-1 protein, expressed by E. coli , with tag free. The total length of Flap endonuclease 1/FEN-1 Protein, Human is 380 a.a., with molecular weight of ~44.5 kDa.

Flap endonuclease 1 (FEN-1) is a structure-specific nuclease with dual enzymatic activities, functioning in both DNA replication and repair processes. In DNA replication, FEN-1 plays a crucial role by cleaving the 5'-overhanging flap structure generated during displacement synthesis, allowing DNA polymerase to navigate the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and tracks to cleave the flap base, creating a nick for subsequent ligation. Additionally, FEN-1 is integral to the long patch base excision repair (LP-BER) pathway, where it cleaves within the apurinic/apyrimidinic (AP) site-terminated flap. Acting as a genome stabilization factor, FEN-1 prevents the equilibration of flaps into structures that may lead to duplications or deletions. Furthermore, the protein exhibits 5'-3' exonuclease activity on nicked or gapped double-stranded DNA and possesses RNase H activity. FEN-1's versatile functions extend to replication and repair processes in rDNA and mitochondrial DNA.

Biological activity has been tested in vitro.

Human

E. coli

Tag Free

P39748 (M1-K380)

2237  [NCBI]

MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK

Approximately 44.5 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 6% trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.