2. Immunology/Inflammation
  3. FLAP
  4. JFD00950

JFD00950 is a human FEN1 inhibitor with an IC50 of 5.5 μM. JFD00950 binds directly to FEN1, alters the secondary structure of this protein, and competitively inhibits its flap cleavage activity by interfering with substrate binding. JFD00950 selectively reduces the viability of cancer cells. JFD00950 can be used in the research of colon cancer and breast cancer.

For research use only. We do not sell to patients.

JFD00950

JFD00950 Chemical Structure

CAS No. : 882278-66-2

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JFD00950 Related Antibodies

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Description

JFD00950 is a human FEN1 inhibitor with an IC50 of 5.5 μM. JFD00950 binds directly to FEN1, alters the secondary structure of this protein, and competitively inhibits its flap cleavage activity by interfering with substrate binding. JFD00950 selectively reduces the viability of cancer cells. JFD00950 can be used in the research of colon cancer and breast cancer[1].

In Vitro

JFD00950 (1.25-50 μM; 20 min) potently inhibits purified human FEN1 flap cleavage activity with an IC50 of 5.5 μM[1].
JFD00950 directly binds to purified human FEN1 protein, as shown by significant quenching of the protein's intrinsic fluorescence at 5 μM[1].
JFD00950 (5 μM; 20 min) acts as a competitive inhibitor of purified human FEN1, increasing the apparent Km for the flap DNA substrate while leaving Vmax unchanged at 5 μM[1].
JFD00950 (3.125-50 μM; 48 h) exhibits selective anti-proliferative activity against cancer cell lines, with the highest potency against DLD-1 colon cancer cells (IC50 = 16.17 μM), and minimal activity against normal HEK-293 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

JFD00950 Related Antibodies

Cell Viability Assay[1]

Cell Line: DLD-1, HT-29, HCT-116, MCF-7, MDAMB-231, HEK-293
Concentration: 3.125, 6.25, 12.5, 25, 50 μM
Incubation Time: 48 h
Result: Inhibited cell growth by 66.60% in DLD-1 cells at 25 μM.
Inhibited cell growth by 50.26% in HT-29 cells at 25 μM.
Inhibited cell growth by 48.8% in HCT-116 cells at 25 μM.
Inhibited cell growth by 46.84% in MCF-7 cells at 25 μM.
Inhibited cell growth by 23.1% in MDAMB-231 cells at 25 μM.
Inhibited cell growth by 20.41% in HEK-293 cells at 25 μM.
Exhibited an IC50 of 16.17 μM for DLD-1 cells.
Molecular Weight

388.03

Formula

C16H6Cl4O3
CAS No.
SMILES

O=C1C(Cl)=C(C(C2=C1C=CC=C2)=O)OC3=CC(Cl)=C(C=C3Cl)Cl
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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JFD00950 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JFD00950882278-66-2JFD 00950JFD-00950FLAP5-lipoxygenase-activating protein5-LO activating proteinFlap endonuclease 1colon cancerHEK-293 cellsflap cleavage activityFEN1DLD-1 colon cancer cellspUC18 plasmid DNAflap DNA substratebreast cancercancer cell linesInhibitorinhibitorinhibit

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