2. Apoptosis Epigenetics Cell Cycle/DNA Damage
  3. Apoptosis PARP
  4. R9-caPep

R9-caPep (RRRRRRRRRCCLGIPEQEY) is a cell-penetrating peptide derived from proliferating cell nuclear antigen (PCNA). R9-caPep selectively blocks the interactions between PCNA and FEN1, as well as between PCNA and LIGI, while preserving the binding of POLD3 to PCNA. R9-caPep interferes with DNA synthesis and homologous recombination-mediated double-strand DNA break repair, inducing S-phase arrest, DNA damage accumulation, and apoptosis. R9-caPep inhibits the growth of tumor volume and weight of neuroblastoma in nude mice. R9-caPep can be used in research related to neuroblastoma and triple-negative breast cancer.

For research use only. We do not sell to patients.

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R9-caPep

R9-caPep Chemical Structure

CAS No. : 1207092-73-6

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R9-caPep Related Antibodies

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Description

R9-caPep (RRRRRRRRRCCLGIPEQEY) is a cell-penetrating peptide derived from proliferating cell nuclear antigen (PCNA). R9-caPep selectively blocks the interactions between PCNA and FEN1, as well as between PCNA and LIGI, while preserving the binding of POLD3 to PCNA. R9-caPep interferes with DNA synthesis and homologous recombination-mediated double-strand DNA break repair, inducing S-phase arrest, DNA damage accumulation, and apoptosis. R9-caPep inhibits the growth of tumor volume and weight of neuroblastoma in nude mice. R9-caPep can be used in research related to neuroblastoma and triple-negative breast cancer[1][2].

In Vitro

R9-caPep (72 h) inhibits the proliferation of various neuroblastoma cell lines, with an IC50 ranging from 10 to 32 mM, and blocks the interaction of proliferating cell nuclear antigen (PCNA)[1].
R9-caPep (0-40 μM, 72 h) significantly reduces the proportion of bromodeoxyuridine-positive cells in SK-N-BE (2) c cells, causes cell DNA replication arrest, inhibits SV40T antigen-mediated DNA replication, induces S-phase cell cycle arrest, and triggers cell apoptosis[1].
R9-caPep (60 μM; 72 h) induces cell death in most tested breast cancer cell lines, with the strongest effects observed in HCC1428 cells (49% increase in cell death) and MDA-MB-231 cells (47% increase in cell death), while exerting no significant effect on BT474 cells[2].
R9-caPep (75 μM, 0-72 h) induces progressive DNA damage and apoptosis in MDA-MB-231 cells. The level of γH2AX increases continuously starting at 24 h, and more than 70% of cells exhibit PARP cleavage by 72 h[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

R9-caPep Related Antibodies

Cell Cycle Analysis[1]

Cell Line: SK-N-BE(2)c cells
Concentration: 0, 20, 40 μM
Incubation Time: 48 h
Result: Caused S-phase cell cycle arrest in neuroblastoma cells, and increased the proportion of cells in the sub-G1 phase.

Cell Viability Assay[2]

Cell Line: HCC1428, MDA-MB-231, BT474, MDA-MB-436, MCF7, SKBR3, MDA-MB-468, HCC1143, HCC1937, UACC893, HCC38, MDA-MB-361, HCC1569
Concentration: 60 μM
Incubation Time: 72 h
Result: Induced increased cell death relative to scrambled control in all tested breast cancer cell lines except BT474.
Increased cell death by 49% in HCC1428 cells.
Increased cell death by 47% in MDA-MB-231 cells.
Showed no significant cell death increase in BT474 cells.

Immunofluorescence[2]

Cell Line: MDA-MB-231
Concentration: 75 μM
Incubation Time: 12, 24, 48, 72 h
Result: Increased the population of cells with high γH2AX levels over the first 24 h, with levels remaining elevated through 72 h.
Increased the percentage of cells with high levels of cleaved PARP progressively over 72 h, rising from 0.2% at 0 h to over 70% by 72 h.
In Vivo

R9-caPep (intratumoral injection, three times per week) inhibits the growth of tumor volume and mass of neuroblastoma in nude mouse xenograft models[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Nude mice (6 weeks, SK-N-BE(2)c cells (5x107 xenografts)) [1]
Dosage: /
Administration: intratumoral injection, three times per week
Result: Inhibited the growth of tumor volume and mass of neuroblastoma in nude mouse xenograft models.
Molecular Weight

2560.02

Formula

C103H183N47O26S2
CAS No.
Sequence

Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Cys-Cys-Leu-Gly-Ile-Pro-Glu-Gln-Glu-Tyr
Sequence Shortening

RRRRRRRRRCCLGIPEQEY
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Storage

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Purity & Documentation
References
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R9-caPep Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

R9-caPep1207092-73-6RRRRRRRRRCCLGIPEQEYApoptosisPARPpoly ADP ribose polymeraseneuroblastomaFEN1PCNAhomologous recombination-mediated double-stranded DNA break repairPOLD3LIGIMDA-MB-231 cellstriple-negative breast cancer cellsDNA synthesiscisplatin-resistant breast cancercell-penetrating peptideInhibitorinhibitorinhibit

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