2. Signaling Pathways
  3. Cell Cycle/DNA Damage
    Epigenetics
  4. PARP
  5. PARP16 Isoform

PARP16

PARP16 is a tail-anchored endoplasmic reticulum (ER) mono-ADP-ribosyltransferase that functions as a key regulator of cellular stress signaling through protein ADP-ribosylation[1]. Mechanistically, PARP16 promotes activation of the unfolded protein response (UPR) by ADP-ribosylating the ER stress sensors PERK (EIF2AK3) and IRE1α (ERN1), thereby enhancing downstream ER stress signaling and maintaining proteostasis under conditions of protein-folding stress[1]. PARP16 enzymatic activity increases during ER stress, and its ADP-ribosylation activity is both necessary and sufficient for efficient PERK and IRE1α activation, establishing PARP16 as an upstream regulator of the UPR pathway[1]. Beyond stress sensing, PARP16 participates in translational control through mono-ADP-ribosylation of ribosomal proteins, linking ER homeostasis to regulation of protein synthesis[2][3]. In disease-relevant models, elevated PARP16 activity enhances ER stress signaling and contributes to pathological cellular responses, whereas PARP16 depletion suppresses UPR activation and reduces disease-associated phenotypes[4][5]. For example, PARP16 knockdown attenuates ER stress-mediated neuronal injury in ischemia/reperfusion-related models, supporting a functional role in stress-induced cell death pathways[5]. Compared with nuclear DNA damage-responsive PARP family members such as PARP1, PARP16 is distinguished by its ER membrane localization and selective regulation of PERK- and IRE1α-dependent UPR signaling rather than canonical DNA repair mechanisms[1]. Therefore, PARP16 represents a valuable experimental target for investigating ER stress biology, UPR regulation, and diseases driven by chronic proteotoxic stress[1][4][5].

PARP16 Related Products (3):

Cat. No. Product Name Effect Purity
  • HY-150221
    DB008
    		Inhibitor 98.47%
    DB008 is potent and selective PARP16 inhibitor with an IC50 value of 0.27 μM, containing an acrylamide electrophilic reagent. DB008 is membrane-permeable and marks PARP16 selectively. DB008 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
  • HY-148754
    PARP10-IN-3
    		Inhibitor 98.72%
    PARP10-IN-3 is a selective mono‐ADP‐ribosyltransferase PARP10 inhibitor with an IC50 of 480 nM for human PARP10. PARP10-IN-3 reveals potent inhibition on PARP2 and PARP15 with IC50s of 1.7 μM for human PARP2 and human PARP15, respectively.
  • HY-148753
    PARP10-IN-2
    		Inhibitor 98.02%
    PARP10-IN-2 is a potent mono‐ADP‐ribosyltransferase PARP10 inhibitor with an IC50 of 3.64 μM for human PARP10. PARP10-IN-2 reveals potent inhibition on PARP2 and PARP15 with IC50s of 27 μM and 11 μM for human PARP2 and human PARP15, respectively.