Follistatin-like 1/FSTL1 Protein, Human (HEK293, His)

Follistatin-like 1 (FSTL1) protein is a secreted glycoprotein that contributes to angiogenesis, immune response regulation, and cell proliferation. It aids in the development of the central nervous system, skeletal system, lungs and ureters. Follistatin-like 1/FSTL1 Protein, Human (HEK293, His) is the recombinant human-derived Follistatin-like 1/FSTL1 protein, expressed by HEK293 , with C-6*His labeled tag.

Follistatin-like 1 (FSTL1) protein is a secreted glycoprotein that plays a role in various physiological processes, including angiogenesis, immune response regulation, cell proliferation, and differentiation. It is involved in the development of the central nervous system, skeletal system, lungs, and ureter. FSTL1 promotes endothelial cell survival, migration, and differentiation into network structures through an AKT-dependent mechanism. It also supports the survival of cardiac myocytes. FSTL1 initiates different signaling cascades by activating various receptors on the cell surface, such as DIP2A, TLR4, or BMP receptors. It forms homodimers and interacts with SCN10A. FSTL1 also interacts with DIP2A, which may act as a cell surface receptor for FSTL1. Additionally, FSTL1 interacts with BMP4 and CD14, with the latter interaction promoting TLR4-mediated signaling cascade.

Measured by its binding ability in a functional ELISA. When Recombinant Human Follistatin-like 1/FSTL1 is coated at 2 μg/mL (100 μL/well) can bind Recombinant human BMP-4. The ED₅₀ for this effect is 40.46 ng/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Human Follistatin-like 1/FSTL1 is coated at 2 μg/mL (100 μL/well) can bind Recombinant human BMP-4. The ED₅₀ for this effect is 40.46 ng/mL.

Human

HEK293

C-6*His

Q12841 (E21-I308)

11167  [NCBI]

Full Length of Mature Protein

EEELRSKSKICANVFCGAGRECAVTEKGEPTCLCIEQCKPHKRPVCGSNGKTYLNHCELHRDACLTGSKIQVDYDGHCKEKKSVSPSASPVVCYQSNRDELRRRIIQWLEAEIIPDGWFSKGSNYSEILDKYFKNFDNGDSRLDSSEFLKFVEQNETAINITTYPDQENNKLLRGLCVDALIELSDENADWKLSFQEFLKCLNPSFNPPEKKCALEDETYADGAETEVDCNRCVCACGNWVCTAMTCDGKNQKGAQTQTEEEMTRYVQELQKHQETAEKTKRVSTKEI

Approximately 48 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.