GCKR Protein, Human (P.pastoris, His)

The GCKR protein plays a critical regulatory role in glucokinase (GCK) by forming an inactive complex and recruiting GCK to the nucleus after meals. Its interaction with GCK is finely tuned by fructose metabolites; binding of GCKR to fructose-6-phosphate increases the affinity for GCK, whereas binding to fructose-1-phosphate decreases the affinity, thereby releasing inhibition. GCKR Protein, Human (P.pastoris, His) is the recombinant human-derived GCKR protein, expressed by P. pastoris , with N-His labeled tag. The total length of GCKR Protein, Human (P.pastoris, His) is 625 a.a., with molecular weight of ~70.7 kDa.

The GCKR protein plays a crucial role in the regulation of glucokinase (GCK) by forming an inactive complex with this enzyme. It acts by facilitating the recruitment of GCK to the nucleus, potentially creating a reservoir of GCK that can be rapidly released into the cytoplasm post-meal. The interaction between GCKR and GCK is finely tuned by fructose metabolites, where GCKR bound to fructose 6-phosphate exhibits increased affinity for GCK. In contrast, GCKR bound to fructose 1-phosphate displays significantly reduced affinity for GCK, leading to the loss of inhibitory effects on GCK activity. This dynamic interplay underscores the sophisticated regulatory mechanism through which GCKR modulates GCK function. Additionally, GCKR interacts with GCK specifically in its fructose 6-phosphate-bound form, highlighting the specificity of the molecular interactions involved in GCK regulation.

Human

P. pastoris

N-His

Q14397 (1M-625Q)

2646  [NCBI]

MPGTKRFQHVIETPEPGKWELSGYEAAVPITEKSNPLTQDLDKADAENIVRLLGQCDAEIFQEEGQALSTYQRLYSESILTTMVQVAGKVQEVLKEPDGGLVVLSGGGTSGRMAFLMSVSFNQLMKGLGQKPLYTYLIAGGDRSVVASREGTEDSALHGIEELKKVAAGKKRVIVIGISVGLSAPFVAGQMDCCMNNTAVFLPVLVGFNPVSMARNDPIEDWSSTFRQVAERMQKMQEKQKAFVLNPAIGPEGLSGSSRMKGGSATKILLETLLLAAHKTVDQGIAASQRCLLEILRTFERAHQVTYSQSPKIATLMKSVSTSLEKKGHVYLVGWQTLGIIAIMDGVECIHTFGADFRDVRGFLIGDHSDMFNQKAELTNQGPQFTFSQEDFLTSILPSLTEIDTVVFIFTLDDNLTEVQTIVEQVKEKTNHIQALAHSTVGQTLPIPLKKLFPSIISITWPLLFFEYEGNFIQKFQRELSTKWVLNTVSTGAHVLLGKILQNHMLDLRISNSKLFWRALAMLQRFSGQSKARCIESLLRAIHFPQPLSDDIRAAPISCHVQVAHEKEQVIPIALLSLLFRCSITEAQAHLAAAPSVCEAVRSALAGPGQKRTADPLEILEPDVQ

Approximately 70.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.