GFRA1/GDNFR-alpha-1 Protein, Canine (HEK293, His)

GFRA1 is a co-receptor that recognizes the GDNF ligand family and is involved in the regulation of proliferation, differentiation and migration of neuronal cells. GFRA1 promotes autophagy through the SRC-AMP-activated protein kinase (AMPK) signaling pathway, thereby contributing to the development of cisplatin-induced chemotherapy resistance to osteosarcoma. GFRA1/GDNFR-alpha-1 Protein, Canine (HEK293, His) is the recombinant canine-derived GFRA1/GDNFR-alpha-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of GFRA1/GDNFR-alpha-1 Protein, Canine (HEK293, His) is 405 a.a., with molecular weight of 53-60 kDa.

Glycosylphosphatidylinositol-linked GDNF (glial cell-derived neurotrophic factor) receptor alpha (GFRA) is a co-receptor that recognizes the GDNF ligand family and plays a critical role in the development and maintenance of the nervous system. GFRA1 is involved in the regulation of proliferation, differentiation and migration of neuron cells. GFRA1 has also been implicated in the progression and metastasis of cancer cells. GFRA1 promotes autophagy through the SRC-AMP-activated protein kinase (AMPK) signaling pathway, thereby contributing to the development of cisplatin-induced chemotherapy resistance to osteosarcoma^[1][2][3].

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Canine GDNF at 1 μg/mL binds Recombinant Canine GFR alpha-1/GDNF R alpha-1. The kD is 4.078 nM.

Canine

HEK293

C-10*His

XP_852087.1 (D25-S429)

609114  [NCBI]

DRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLTSGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRVVPFLPDVLQQVEHIPKGNNCLDAAKACNLDDTCKKYRSAYITPCTTSMSNEVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEEREKPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSASSCLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEECLKFLNFFKDNTCLKNAIQAFGNGSDVTVWQPALPVQTTTATTTTAFRAKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNMSGSTHLCLSDRDYEKNGLSGPS

Approximately 53-60 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.