HGFA/HGF Activator Protein, Mouse (HEK293, His)

HGFA/HGF activating proteins activate HGF by converting HGF into its heterodimeric form, which is critical for cell growth, development, and tissue repair. It induces cellular responses such as proliferation, migration, and differentiation. HGFA/HGF Activator Protein, Mouse (HEK293, His) is the recombinant mouse-derived HGFA/HGF Activator protein, expressed by HEK293 , with C-6*His labeled tag.

HGFA/HGF Activator Protein is responsible for activating hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form. This heterodimer consists of a short chain and a long chain that are linked together by a disulfide bond. HGFA/HGF Activator Protein plays a crucial role in various biological processes, including cell growth, development, and tissue repair. Its activation of HGF leads to the induction of cellular responses such as cell proliferation, migration, and differentiation. By modulating HGF activity, HGFA/HGF Activator Protein contributes to the regulation of cell-matrix adhesion, cell-cell adhesion, and cell morphology. It interacts with various proteins, including THSD1, PTK2/FAK1, TLN1, and VCL, and its association with CTNNA1 is essential for its localization to cell-cell junctions and the regulation of E-cadherin expression. Moreover, HGFA/HGF Activator Protein forms a complex with APBB1IP, NRAP, TLN1, CTNNB1, SYNM, SORBS1, and CTNNA1, and it triggers conformational changes when binding to ACTN4. Its multifaceted interactions and activities highlight its importance in cellular processes and underline its potential therapeutic applications.

Measured in a cell proliferation assay using hepG2 human hepatocellular carcinoma cells. The ED₅₀ for this effect is 0.951 μg/mL, corresponding to a specific activity is 5.126×10³ U/mg.

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  Measured in a cell proliferation assay using hepG2 human hepatocellular carcinoma cells. The ED₅₀ for this effect is 0.951 μg/mL, corresponding to a specific activity is 5.126×10³ U/mg.

Mouse

HEK293

C-6*His

Q9R098 (Q35-S653)

54426  [NCBI]

Full Length of Mature Protein

QAGRNHTEPPGPNVTATPVTPTIPVISGNVSTSTESAPAAETEGPQSERYPPPSSSSPPGGQVLTESGQPCRFPFRYGGRMLHSCTSEGSAYRKWCATTHNYDRDRAWGYCAEVTLPVEGPAILDPCASGPCLNGGTCSSTHDHGSYHCSCPLAFTGKDCGTEKCFDETRYEYFEVGDHWARVSEGHVEQCGCMEGQARCEDTHHTACLSSPCLNGGTCHLIVGTGTSVCTCPLGYAGRFCNIVPTEHCFLGNGTEYRGVASTAASGLSCLAWNSDLLYQELHVDSVAAAVLLGLGPHAYCRNPDKDERPWCYVVKDNALSWEYCRLTACESLARVHSQTPEILAALPESAPAVRPTCGKRHKKRTFLRPRIIGGSSSLPGSHPWLAAIYIGNSFCAGSLVHTCWVVSAAHCFANSPPRDSITVVLGQHFFNRTTDVTQTFGIEKYVPYTLYSVFNPNNHDLVLIRLKKKGERCAVRSQFVQPICLPEAGSSFPTGHKCQIAGWGHMDENVSSYSNSLLEALVPLVADHKCSSPEVYGADISPNMLCAGYFDCKSDACQGDSGGPLVCEKNGVAYLYGIISWGDGCGRLNKPGVYTRVANYVDWINDRIRPPKRPVATS

Approximately 80-100 kDa, based on SDS-PAGE under reducing conditions.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, 2 mM CaCl₂, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, 2 mM CaCl₂, pH 8.0, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.