IL-15R alpha Protein, Human (HEK293, Fc)

IL-15R alpha Protein, a proven high-affinity receptor for interleukin-15, signals in both cis and trans. In neutrophils, it activates SYK kinase, crucial for IL-15-induced phagocytosis in a SYK-dependent manner. Different isoforms may introduce variations in signal transduction. Notably, IL-15R alpha Protein, while having high affinity, does not directly bind to IL15. IL-15R alpha Protein, Human (HEK293, Fc) is the recombinant human-derived IL-15R alpha protein, expressed by HEK293, with C-hFc labeled tag.

IL-15R alpha Protein stands out as a high-affinity receptor for interleukin-15, as evidenced by studies. This receptor exhibits the remarkable capability to signal both in cis and trans, where IL15R from one subset of cells presents interleukin-15 to neighboring IL2RG-expressing cells (By similarity). Notably, in neutrophils, IL-15R alpha Protein binds to and activates the kinase SYK in response to interleukin-15 stimulation, playing a critical role in IL-15-induced phagocytosis in a SYK-dependent manner. It's important to note that the expression of different isoforms may introduce variations or interference with signal transduction processes. Despite its high affinity for interleukin-15, it is specified that IL-15R alpha Protein does not directly bind to IL15.

Measured by its binding ability in a functional ELISA. Immobilized Human IL-15 R alpha, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-15, Fc, Avitag (QC tested).

Human

HEK293

C-hFc

Q13261-1 (I31-T205)

3601

Extracellular Domain

ITCPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDPALVHQRPAPPSTVTTAGVTPQPESLSPSGKEPAASSPSSNNTAATTAAIVPGSQLMPSKSPSTGTTEISSHESSHGTPSQTTAKNWELTASASHQPPGVYPQGHSDTT

Approximately 58-66 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with 10% trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.