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  6. IL-1RAcP/IL-1 R3 Protein, Cynomolgus (HEK293, His)

IL-1RAcP/IL-1 R3 Protein, Cynomolgus (HEK293, His)

Cat. No.: HY-P77412
COA Handling Instructions

IL-1RAcP is a co-receptor for IL1RL2, IL1R1 and IL1RL1 in the IL-36, IL-1 and IL-33 signaling system, respectively. IL-1RAcP is an essential component of the IL-1 receptor and plays an essential role in the inflammatory process. IL-1RAcP/IL-1 R3 Protein, Cynomolgus (HEK293, His) is a recombinant cynomolgus IL-1RAcP protein with a His tag at the C-terminus and is expressed in HEK293 cells.

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Description

IL-1RAcP is a co-receptor for IL1RL2, IL1R1 and IL1RL1 in the IL-36, IL-1 and IL-33 signaling system, respectively. IL-1RAcP is an essential component of the IL-1 receptor and plays an essential role in the inflammatory process[1][2]. IL-1RAcP/IL-1 R3 Protein, Cynomolgus (HEK293, His) is a recombinant cynomolgus IL-1RAcP protein with a His tag at the C-terminus and is expressed in HEK293 cells.

Background

Interleukin-1 receptor accessory protein (IL-1RAcP), also known as IL-1 R3, belongs to the immunoglobulin (Ig) superfamily. IL-1RAcP can be detected in liver, skin, placenta, thymus and lung.
The cynomolgus IL-1RAcP protein shares 99.30% a.a sequence identity with human, 88.60% identity with rat and 88.42% identity with mouse.
IL-1RAcP contains three Ig-like domains and a cytoplasmic domain named Toll/IL-1 receptor (TIR) (position: 403-546 a.a), which has been implicated in signal transduction. IL-1RAcP also contains a single transmembrane region. IL-1RAcP is a co-receptor for IL1RL2, IL1R1 and IL1RL1 in the IL-36, IL-1 and IL-33 signaling system, respectively. IL-1RAcP is an indispensible molecule in the IL-1 receptor signal transduction complex, links events at the plasma membrane level to downstream signaling pathways, mediates interleukin-1-dependent transcription factor activation and gene expression.
IL-1RAcP plays an essential role in the inflammatory process and can be used for the research of obesity, diabetics, endometriosis, atherosclerosis, and so on[1][2][3].

Biological Activity

Measured by its ability to inhibit IL-1 alpha -induced IL-8 secretion in HepG2 human hepatocellular carcinoma cells. The ED50 for this effect is 1.163 µg/mL in the presence of 1 μg/mL of recombinant human IL-1 R2 and 50 pg/mL rhIL-1 alpha, corresponding to a specific activity is 619.963 U/mg.

  • Measured by its ability to inhibit IL-1 alpha -induced IL-8 secretion in HepG2 human hepatocellular carcinoma cells. The ED50 for this effect is 1.163 µg/mL in the presence of 1 μg/mL of recombinant human IL-1 R2 and 50 pg/mL rhIL-1 alpha, corresponding to a specific activity is 619.963 U/mg.
Species

Cynomolgus

Source

HEK293

Tag

C-His

Accession

P59822-1/NP_001028132.1 (S21-E359)

Gene ID

574387  [NCBI]

Molecular Construction
N-term
IL-1RAcP (S21-E359)
Accession # P59822-1/NP_001028132.1
His
C-term
Synonyms
Interleukin-1 Receptor Accessory Protein; IL-1RAcP; Interleukin-1 Receptor 3; IL-1R3; IL1RAP; C3orf13
AA Sequence

SERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKFNYSTAHSAGLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNSPMKLPVHKLYIEYGIQRITCPNVDGYFPSSVKPTITWYMGCYKIQNFNNVIPEGMNLSFLIAFISNNGNYTCVVTYPENGRTFHLTRTLTVKVVGSPKNAVPPVIHSPNDHVVYEKEPGEELLIPCTVYFSFLMDSRNEVWWTIDGKKPDDIPIDVTINESISHSRTEDETRTQILSIKKVTSEDLKRSYVCHARSAKGEVAKAATVKQKVPAPRYTVE

Molecular Weight

Approximately 55-70 kDa due to the glycosylation

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

IL-1RAcP/IL-1 R3 Protein, Cynomolgus (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-1RAcP/IL-1 R3 Protein, Cynomolgus (HEK293, His)
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HY-P77412
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