Copy Link Print Information

IL-4 Protein, Human

63 Cited Publications
Customer Review

Based on 63 publication(s) in Google Scholar

IL-4 Protein is an endogenous agonist of IL-4 receptor (IL-4R), which works through the JAK1/JAK3-STAT6 signaling pathway. It can cooperate with SCF to promote the proliferation of human intestinal mast cells (ED50=100 pg/mL) and enhance the release of mediators such as histamine mediated by IgE receptor. IL-4 Protein can inhibit the production of IL-8 by human monocytes and regulate the secretion of endothelial cell factors. It is mainly used for IL-4R targeted therapy research of tumors (such as malignant pleural mesothelioma) and allergic diseases. IL-4 Protein, Human is a recombinant protein of human IL-4, expressed by E. coli, with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IL-4 Protein, Human
  • Experimental Validation Results for IL-4 Protein, Human
  • Experimental Validation Results for IL-4 Protein, Human
  • Experimental Validation Results for IL-4 Protein, Human
  • Experimental Validation Results for IL-4 Protein, Human
  • Experimental Validation Results for IL-4 Protein, Human
  • Experimental Validation Results for IL-4 Protein, Human
  • Experimental Validation Results for IL-4 Protein, Human
  • IL-4 Protein, Human Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

IL-4 Protein is an endogenous agonist of IL-4 receptor (IL-4R), which works through the JAK1/JAK3-STAT6 signaling pathway. It can cooperate with SCF to promote the proliferation of human intestinal mast cells (ED50=100 pg/mL) and enhance the release of mediators such as histamine mediated by IgE receptor. IL-4 Protein can inhibit the production of IL-8 by human monocytes and regulate the secretion of endothelial cell factors. It is mainly used for IL-4R targeted therapy research of tumors (such as malignant pleural mesothelioma) and allergic diseases. IL-4 Protein, Human is a recombinant protein of human IL-4, expressed by E. coli, with tag free.

Background

IL-4 Protein is a cytokine of the interleukin-4 family, a glycoprotein with an α-helical structure, and an endogenous agonist of the IL-4 receptor (IL-4R), binding to the IL-4Rα and γc chains. IL-4 Protein was originally called B cell growth factor BSF1 and was described in the supernatant of activated EL-4 thymoma cells to costimulate with subservient concentrations of anti-IgM. IL-4 Protein exerts biological activity through the JAK1/JAK3-STAT6 signaling pathway, and can promote the proliferation of human intestinal mast cells (ED50=100 pg/mL) in coordination with stem cell factor (SCF), enhance the release of histamine, leukotriene C4, and IL-5 mediated by IgE receptors, inhibit the secretion of IL-8 mRNA and protein by human monocytes stimulated by LPS/TNF-α/IL-1β, and selectively enhance the secretion of IL-6 and inhibit IL-8 by human umbilical vein endothelial cells. Its mechanism of action involves regulating the expression of cell membrane receptors and the activation of downstream transcription factors. It is mainly used in the study of immune regulation mechanisms and targeted therapy of allergic diseases (such as asthma) and tumors (such as malignant pleural mesothelioma), especially the application of IL-4R-targeted cytotoxin fusion proteins (such as IL-4-PE, cpIL-4-PE) in tumor ablation. IL-4 Protein plays an important role in regulating inflammation and immune response. IL-4 Protein induces naive helper T cells to differentiate into Th2 cells. While IL-4 Protein binds to the IL-4 receptor, the IL-4 receptor also binds to another cytokine, IL-13, which may explain the overlapping functions of IL-4 and IL-13[1][2][3][4][5].

Verified Bioactivity

The cell proliferation assay using TF-1 human erythroleukemic cells has an ED50 value of ≤450 pg/mL.

Results
  • Experimental Validation Results for IL-4 Protein, Human
    Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is 0.2989 ng/mL., corresponding to a specific activity is 3.35×106 U/mg

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P05112-1 (H25-S153)

  • Gene ID
    3565  [NCBI]
  • Molecular Construction
    • N-term
    • IL-4 (H25-S153)
      Accession # P05112-1
    • C-term
  • Protein Length

    Full Length of Isoform-1 Mature Protein

  • Synonyms

    Interleukin-4; IL-4; B-Cell Stimulatory Factor 1; BSF-1; Binetrakin; Lymphocyte Stimulatory Factor 1; Pitrakinra; IL4

  • AA Sequence

    HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

  • Predicted Molecular Mass

    15.1 kDa

  • Molecular Weight

    Approximately 13-14 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

    • Experimental Validation Results for IL-4 Protein, Human
      ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 7.2.
2.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 8.0.
3.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 8.0, 5% trehalose.
4.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Bischoff SC, et al. IL-4 enhances proliferation and mediator release in mature human mast cells. Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):8080-5. [Content Brief]

[2]. Standiford TJ, et al. IL-4 inhibits the expression of IL-8 from stimulated human monocytes. J Immunol. 1990 Sep 1;145(5):1435-9. [Content Brief]

[3]. Chen C C, et al. TGF-β1, IL-10 and IL-4 differentially modulate the cytokine-induced expression of IL-6 and IL-8 in human endothelial cells. cytokine, 1996, 8(1): 58-65.

[4]. Ishige K, et al. Potent in vitro and in vivo antitumor activity of interleukin-4-conjugated Pseudomonas exotoxin against human biliary tract carcinoma. Int J Cancer. 2008 Dec 15;123(12):2915-22. [Content Brief]

[5]. Beseth BD, et al. Interleukin-4 receptor cytotoxin as therapy for human malignant pleural mesothelioma xenografts. Ann Thorac Surg. 2004 Aug;78(2):436-43; discussion 436-43. [Content Brief]

View More

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

  • ≥ 95%, as determined by reducing SDS-PAGE.

  • Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is 0.2989 ng/mL., corresponding to a specific activity is 3.35×106 U/mg

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is 0.2989 ng/mL., corresponding to a specific activity is 3.35×106 U/mg

  • 1/16

    IL-4 Protein, Human purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jan;12(2):e2406865. [Abstract]

    Customer Validation
     M2‐like macrophage biomarkers were detected in THP‐1 macrophages treated with IL4 (30 ng/mL; 72 h) via qRT‐PCR.

  • 1/16

    IL-4 Protein, Human purchased from MedChemExpress. Usage Cited in: Allergy. 2025 Sep 15. [Abstract]

    Customer Validation
     Histogram showing Ach concentrations in the basolateral medium of ALI cultures under the following conditions: Untreated, α-NETA-treated, and IL-4 (5 ng/mL; 21 days) /IL-5/IL-13 treatment with or without α-NETA, as measured by ELISA.

  • 1/16

    IL-4 Protein, Human purchased from MedChemExpress. Usage Cited in: Cells. 2025 Jul 1;14(13):1004. [Abstract]

    Customer Validation
     HK2 mRNA levels were measured after 48 h of stimulation with IL-4.（20 μg/mL; 48 h）

  • 1/16

    IL-4 Protein, Human purchased from MedChemExpress. Usage Cited in: Arthritis Res Ther. 2025 Nov 18;27(1):216. [Abstract]

    Customer Validation
     Western blot was used to determine the relative expression of Omentin-1 protein in Raw264.7 cells. Omentin-1 promoted the transformation of Raw264.7 cells into the M2 phenotype while inhibiting the conversion to the M1 phenotype. Raw264.7 cells were induced to differentiate into M1 macrophages by LPS (10 pg/mL) and IFN-γ (IFN-gamma Protein, Human (CHO); 20 ng/mL) for 24 h, while they were induced to differentiate into M2 macrophages by IL-4 (20 ng/mL) and IL-13 (20 ng/mL). After 24 h, Omentin-1 was added to the cell culture medium, and the incubation was continued for an additional 24 h

  • 1/16

    IL-4 Protein, Human purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Oct;9(29):e2202039. [Abstract]

    Customer Validation
     FACS analysis of IL‐4‐induced (50 ng/mL; 48 h) M2‐like macrophages after incubation with anti‐F4/80 and anti‐CD206 antibodies.

  • 1/16

    IL-4 Protein, Human purchased from MedChemExpress. Usage Cited in: Cells. 2022 Aug 28;11(17):2674. [Abstract]

    Customer Validation
     IL-4 (20 ng/mL; 24 h) The expression of M2 surface markers CD163 in THP-1 was analyzed by flow cytometry.

  • IL-4 Protein, Human Product Image
    1/16
  • Experimental Validation Results for IL-4 Protein, Human
  • Experimental Validation Results for IL-4 Protein, Human
  • Experimental Validation Results for IL-4 Protein, Human
  • Experimental Validation Results for IL-4 Protein, Human
  • Experimental Validation Results for IL-4 Protein, Human
  • Experimental Validation Results for IL-4 Protein, Human
  • Experimental Validation Results for IL-4 Protein, Human
  • Experimental Validation Results for IL-4 Protein, Human
  • IL-4 Protein, Human Product Image

  • IL-4 Protein, Human purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jan;12(2):e2406865. [Abstract]

    M2‐like macrophage biomarkers were detected in THP‐1 macrophages treated with IL4 (30 ng/mL; 72 h) via qRT‐PCR.

  • IL-4 Protein, Human purchased from MedChemExpress. Usage Cited in: Allergy. 2025 Sep 15. [Abstract]

    Histogram showing Ach concentrations in the basolateral medium of ALI cultures under the following conditions: Untreated, α-NETA-treated, and IL-4 (5 ng/mL; 21 days) /IL-5/IL-13 treatment with or without α-NETA, as measured by ELISA.

  • IL-4 Protein, Human purchased from MedChemExpress. Usage Cited in: Cells. 2025 Jul 1;14(13):1004. [Abstract]

    HK2 mRNA levels were measured after 48 h of stimulation with IL-4.（20 μg/mL; 48 h）

  • IL-4 Protein, Human purchased from MedChemExpress. Usage Cited in: Arthritis Res Ther. 2025 Nov 18;27(1):216. [Abstract]

    Western blot was used to determine the relative expression of Omentin-1 protein in Raw264.7 cells. Omentin-1 promoted the transformation of Raw264.7 cells into the M2 phenotype while inhibiting the conversion to the M1 phenotype. Raw264.7 cells were induced to differentiate into M1 macrophages by LPS (10 pg/mL) and IFN-γ (IFN-gamma Protein, Human (CHO); 20 ng/mL) for 24 h, while they were induced to differentiate into M2 macrophages by IL-4 (20 ng/mL) and IL-13 (20 ng/mL). After 24 h, Omentin-1 was added to the cell culture medium, and the incubation was continued for an additional 24 h

  • IL-4 Protein, Human purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Oct;9(29):e2202039. [Abstract]

    FACS analysis of IL‐4‐induced (50 ng/mL; 48 h) M2‐like macrophages after incubation with anti‐F4/80 and anti‐CD206 antibodies.

  • IL-4 Protein, Human purchased from MedChemExpress. Usage Cited in: Cells. 2022 Aug 28;11(17):2674. [Abstract]

    IL-4 (20 ng/mL; 24 h) The expression of M2 surface markers CD163 in THP-1 was analyzed by flow cytometry.