1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-5
  5. IL-5 Protein, Mouse (sf9, His)

IL-5 Protein, Mouse (sf9, His)

Cat. No.: HY-P73219
Handling Instructions

IL-5 protein is expressed by T lymphocytes and NK cells and regulates eosinophils, affecting their survival, differentiation and chemotaxis. In addition, IL-5 stimulates immunoglobulin production, growth, and differentiation of activated and resting B cells. IL-5 Protein, Mouse (sf9, His) is the recombinant mouse-derived IL-5 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of IL-5 Protein, Mouse (sf9, His) is 133 a.a., with molecular weight of ~18 kDa.

For research use only. We do not sell to patients.

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Description

IL-5 protein is expressed by T lymphocytes and NK cells and regulates eosinophils, affecting their survival, differentiation and chemotaxis. In addition, IL-5 stimulates immunoglobulin production, growth, and differentiation of activated and resting B cells. IL-5 Protein, Mouse (sf9, His) is the recombinant mouse-derived IL-5 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of IL-5 Protein, Mouse (sf9, His) is 133 a.a., with molecular weight of ~18 kDa.

Background

IL-5 Protein, a homodimeric cytokine predominantly expressed by T-lymphocytes and NK cells, plays a pivotal role in the regulation of eosinophils by influencing their survival, differentiation, and chemotaxis. Additionally, IL-5 acts on both activated and resting B-cells, stimulating immunoglobulin production, growth, and differentiation. Mechanistically, the biological effects of IL-5 are mediated through a receptor complex composed of the IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Upon binding to the receptor, IL-5 triggers the activation of various kinases, including LYN, SYK, and JAK2, thus propagating signals through the RAS-MAPK and JAK-STAT5 pathways (By similarity). Structurally, IL-5 forms a homodimer that is disulfide-linked and interacts with its receptor components IL5RA and CSF2RB, highlighting the specificity and complexity of its molecular interactions in orchestrating immune responses.

Species

Mouse

Source

Sf9 insect cells

Tag

C-His

Accession

P04401 (M1-G133)

Gene ID
Molecular Construction
N-term
IL-5 (M1-G133)
Accession # P04401
His
C-term
Synonyms
Interleukin-5; IL-5; EDF; Eo-CSF; TRF
Molecular Weight

Approximately 18 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

IL-5 Protein, Mouse (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IL-5 Protein, Mouse (sf9, His)
Cat. No.:
HY-P73219
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