Inhibin beta C chain/INHBC Protein, Human (HEK293, His)

The inhibin beta C chain (INHBC) protein complexly regulates the inhibin and activin systems and balances follicle-stimulating hormone secretion. Its key roles span a variety of physiological processes, including hormone secretion, cell development, insulin release, and bone growth, depending on the specific subunit composition. Inhibin beta C chain/INHBC Protein, Human (HEK293, His) is the recombinant human-derived Inhibin beta C chain/INHBC protein, expressed by HEK293 , with C-6*His labeled tag.

INHBC protein stands at the nexus of intricate regulatory pathways as part of the inhibin and activin systems, orchestrating the dual actions of inhibiting and activating the secretion of follitropin by the pituitary gland. The multifaceted roles of inhibins and activins, in which INHBC plays a pivotal role, span diverse physiological processes encompassing hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development, and bone growth, contingent upon the specific subunit composition. In this complex interplay, inhibins, exemplified by heterodimers like Inhibin A and Inhibin B, emerge as counterparts opposing the functions of activins. Structurally, INHBC exists in a homodimeric or heterodimeric configuration through association with alpha and beta subunits, intricately linked by one or more disulfide bonds. Notably, inhibins manifest as heterodimers comprising one alpha and one beta subunit, while activins, whether homodimers or heterodimers, exclusively consist of beta subunits. This structural diversity underlines the nuanced regulatory mechanisms orchestrated by INHBC in shaping a comprehensive and finely tuned physiological landscape.

Human

HEK293

C-6*His

P55103 (T19-S352)

3626  [NCBI]

Full Length of Mature Protein

TPRAGGQCPACGGPTLELESQRELLLDLAKRSILDKLHLTQRPTLNRPVSRAALRTALQHLHGVPQGALLEDNREQECEIISFAETGLSTINQTRLDFHFSSDRTAGDREVQQASLMFFVQLPSNTTWTLKVRVLVLGPHNTNLTLATQYLLEVDASGWHQLPLGPEAQAACSQGHLTLELVLEGQVAQSSVILGGAAHRPFVAARVRVGGKHQIHRRGIDCQGGSRMCCRQEFFVDFREIGWHDWIIQPEGYAMNFCIGQCPLHIAGMPGIAASFHTAVLNLLKANTAAGTTGGGSCCVPTARRPLSLLYYDRDSNIVKTDIPDMVVEACGCS

Approximately 42-58 kDa & 32-40 kDa & 14-17 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 10% trehalose, 2% mannitol, 0.05% Tween 80, pH 8.2.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.