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  5. Kanamycin kinase type II/NEO protein, Klebsiella pneumoniae

Kanamycin kinase type II/NEO protein, Klebsiella pneumoniae

Cat. No.: HY-P70228
COA Handling Instructions

The Kanamycin kinase type II/NEO protein imparts resistance to aminoglycoside antibiotics, including kanamycin, neomycin, paromomycin, ribostamycin, butirosin, and gentamicin B. It counteracts their inhibitory effects, showcasing the protein's versatility in mitigating the impact of various antibiotics and enabling the organism to thrive in their presence. Kanamycin kinase type II/NEO protein, Klebsiella pneumoniae is the recombinant Kanamycin kinase type II/NEO protein, expressed by E. coli , with tag free. The total length of Kanamycin kinase type II/NEO protein, Klebsiella pneumoniae is 264 a.a., with molecular weight of 26-30 kDa.

For research use only. We do not sell to patients.

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Description

The Kanamycin kinase type II/NEO protein imparts resistance to aminoglycoside antibiotics, including kanamycin, neomycin, paromomycin, ribostamycin, butirosin, and gentamicin B. It counteracts their inhibitory effects, showcasing the protein's versatility in mitigating the impact of various antibiotics and enabling the organism to thrive in their presence. Kanamycin kinase type II/NEO protein, Klebsiella pneumoniae is the recombinant Kanamycin kinase type II/NEO protein, expressed by E. coli , with tag free. The total length of Kanamycin kinase type II/NEO protein, Klebsiella pneumoniae is 264 a.a., with molecular weight of 26-30 kDa.
Background

The Kanamycin kinase type II/NEO protein confers resistance to a spectrum of aminoglycoside antibiotics, including kanamycin, neomycin, paromomycin, ribostamycin, butirosin, and gentamicin B. Its role involves mitigating the impact of these antibiotics, allowing the organism to withstand their inhibitory effects. This resistance mechanism highlights the versatility of the Kanamycin kinase type II/NEO protein, as it counteracts multiple members of the aminoglycoside antibiotic class, contributing to the organism's ability to thrive in the presence of these antimicrobial agents.
Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Species

Others
Source

E. coli
Tag

Tag Free
Accession

P00552 (M1-F264)
Gene ID

59690385
Molecular Construction
N-term
NEO (M1-F264)
Accession # P00552
C-term
Synonyms
rKlAminoglycoside 3'-phosphotransferase; Aminoglycoside 3'-phosphotransferase; APH(3')-II; APH(3')II; Kanamycin kinase type II; Neomycin-kanamycin phosphotransferase type II; neo
AA Sequence

MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF

Molecular Weight

26-30 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Solution.
Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 6%Trehalose, 4%Mannitol, 0.05%Tween80, pH 8.0.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation

COA (219 KB)

Handling Instructions (2659 KB)

Kanamycin kinase type II/NEO protein, Klebsiella pneumoniae Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Kanamycin kinase type II/NEO protein, Klebsiella pneumoniae
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