NCAM-1/CD56 Protein, Mouse (HEK293, His)

NCAM-1/CD56 protein is an important cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, and neurite outgrowth. It interacts with MDK and forms a complex with SLC39A6 and SLC39A10, complexly regulating NCAM-1 phosphorylation. NCAM-1/CD56 Protein, Mouse (HEK293, His) is the recombinant mouse-derived NCAM-1/CD56 protein, expressed by HEK293 , with C-His, C-10*His labeled tag.

The NCAM-1/CD56 protein takes on a pivotal role as a cell adhesion molecule, participating in neuron-neuron adhesion, neurite fasciculation, and the outgrowth of neurites. It engages in interactions with MDK and forms a complex with SLC39A6, SLC39A10, and itself. This complex intricately regulates NCAM-1 phosphorylation and facilitates its integration into focal adhesion complexes during epithelial-to-mesenchymal transition, as observed in studies. The versatile involvement of NCAM-1 in cell adhesion and signaling processes highlights its significance in mediating complex cellular interactions critical for neuronal development and transitions between epithelial and mesenchymal states.

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a cells. The ED₅₀ this effect is 0.1-0.25 μg/mL.

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  Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a cells. The ED₅₀ for this effect is 0.1865 μg/mL, corresponding to a specific activity is 5.36×10³ units/mg.

Mouse

HEK293

C-10*His

P13595 (L20-T711)

17967  [NCBI]

Extracellular Domain

LQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRISVVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDKNDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKTLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEFQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEESWHFKWYDAKEANMEGIVTIMGLKPETRYSVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTAGLST

approximately 95-130 kDa due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 500 mM NaCl, pH 7.5.
2.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 500 mM NaCl, pH 7.5, 5% trehalose, 5% mannitol, 0.01% Tween 80.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.