1. Recombinant Proteins
  2. CD Antigens Receptor Proteins
  3. NK Cell CD Proteins
  4. CD337/NCR3
  5. NKp30/NCR3 Protein, Cynomolgus/Rhesus Macaque (HEK293, His)

NKp30/NCR3 Protein, Cynomolgus/Rhesus Macaque (HEK293, His)

Cat. No.: HY-P77452
COA Handling Instructions

NKp30/NCR3 protein is a cell membrane receptor on natural killer (NK) cells that activates cytotoxicity by binding to ligands such as BAG6 and NCR3LG1, stimulating NK cells to respond against neighboring cells (including tumor cells) expressing these ligands. NKp30/NCR3 Protein, Cynomolgus/Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived NKp30/NCR3 protein, expressed by HEK293 , with C-His labeled tag. The total length of NKp30/NCR3 Protein, Cynomolgus/Rhesus Macaque (HEK293, His) is 117 a.a., with molecular weight of ~23-32 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

NKp30/NCR3 protein is a cell membrane receptor on natural killer (NK) cells that activates cytotoxicity by binding to ligands such as BAG6 and NCR3LG1, stimulating NK cells to respond against neighboring cells (including tumor cells) expressing these ligands. NKp30/NCR3 Protein, Cynomolgus/Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived NKp30/NCR3 protein, expressed by HEK293 , with C-His labeled tag. The total length of NKp30/NCR3 Protein, Cynomolgus/Rhesus Macaque (HEK293, His) is 117 a.a., with molecular weight of ~23-32 kDa.

Background

NKp30/NCR3 protein, serving as a cell membrane receptor on natural killer (NK) cells, becomes activated upon binding extracellular ligands such as BAG6 and NCR3LG1. Upon ligand binding, NKp30/NCR3 stimulates NK cell cytotoxicity against neighboring cells expressing these ligands, thereby exerting control over NK cell cytotoxicity against tumor cells. The engagement of NCR3 by BAG6 not only enhances NK cell-mediated killing of myeloid dendritic cells (DCs) that failed to acquire a mature phenotype but also promotes DC maturation. This occurs through the release of TNFA and IFNG by NK cells, further contributing to the maturation process. In its unliganded form, NKp30/NCR3 forms homodimers and interacts with CD3Z, as well as with and is activated by binding to both NCR3LG1 and BAG6, unraveling its intricate roles in regulating immune responses, NK cell cytotoxicity, and DC maturation.

Biological Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H6 is immobilized at 0.5 µg/mL (100 µL/well) can bind Recombinant Cynomolgus NKp30/NCR3. The ED50 for this effect is 59.89 ng/mL.

  • Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H6 is immobilized at 0.5 µg/mL (100 µL/well) can bind Recombinant Cynomolgus NKp30/NCR3. The ED50 for this effect is 59.89 ng/mL.
Species

Rhesus Macaque

Source

HEK293

Tag

C-His

Accession

XP_005553604 (L19-G135)

Gene ID
Molecular Construction
N-term
NKp30 (L19-G135)
Accession # XP_005553604
His
C-term
Synonyms
NCR3 Protein; Natural Cytotoxicity Triggering Receptor 3; NCR3; CD337; 1C7; LY117
AA Sequence

LWVSQPPEIRTLEGSSAFLPCSFNASQGRLAIGSVTWFRDEVAPGKEVRNGTPEFRGRLAPLSSSRFLRDHQAELHIWDVRGHDAGIYVCRVEVLGLGVGTGNGTRLVVEKEYPQLG

Molecular Weight

The protein migrates as an approximately 23-32 kDa band under reducing SDS-PAGE due to glycosylation.

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

NKp30/NCR3 Protein, Cynomolgus/Rhesus Macaque (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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NKp30/NCR3 Protein, Cynomolgus/Rhesus Macaque (HEK293, His)
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HY-P77452
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