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PD-1 Protein, Human (HEK293, His)

2 Cited Publications
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Based on 2 publication(s) in Google Scholar

PD-1 Protein, Human (HEK293, His) suppress activating signals from the T cell receptor when bound by either of its ligands, programmed death-ligand 1 (PD-L1) or PD-L2.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for PD-1 Protein, Human (HEK293, His)
  • Experimental Validation Results for PD-1 Protein, Human (HEK293, His)
  • Experimental Validation Results for PD-1 Protein, Human (HEK293, His)
  • Experimental Validation Results for PD-1 Protein, Human (HEK293, His)
  • Experimental Validation Results for PD-1 Protein, Human (HEK293, His)
  • Experimental Validation Results for PD-1 Protein, Human (HEK293, His)
  • PD-1 Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

PD-1 Protein, Human (HEK293, His) suppress activating signals from the T cell receptor when bound by either of its ligands, programmed death-ligand 1 (PD-L1) or PD-L2.

Background

Programmed death-1 (PD-1) is a receptor on T cells that has been shown to suppress activating signals from the T cell receptor when bound by either of its ligands, programmed death-ligand 1 (PD-L1) or PD-L2. When PD-1 expressing T cells contact cells expressing its ligands, functional activities in response to antigenic stimuli, including proliferation, cytokine secretion, and cytotoxicity are reduced[1].

Verified Bioactivity

1. Measured by its ability to inhibit proliferation of HT-29 cells. The ED50 this effect is 0.1452 μg/mL, corresponding to a specific activity is 6.89×103 units/mg.
2. Loaded Pembrolizumab (HY-P9902) on AHC2 biosensor, can bind PD-1 Protein, Human (143a.a, HEK293, His) with an affinity constant of 1.898E-09 M as determined in BLI assay.
3. Loaded Ivonescimab (HY-P99675) on AHC2 biosensor, can bind PD-1 Protein, Human (143a.a, HEK293, His) with an affinity constant of 5.804E-08 M as determined in BLI assay.
4. Loaded Rosnilimab (HY-P9997) on AHC2 biosensor, can bind PD-1 Protein, Human (143a.a, HEK293, His) with an affinity constant of 2.896E-09 M as determined in BLI assay.
5. Measured by its binding ability in a functional ELISA. Immobilized Human PD-1 Protein at 2 µg/mL (100µL/well) can bind Biotinylated Human PD-L1 Protein. The ED50 for this effect is 100-500 ng/mL.
6. Immobilized PD-1 Protein, Human can bind IBI-363 (HY-P991740). The ED50 for this effect is 4.714 ng/mL.
7. Human PD-L1 Protein （HY-P73361）immobilized on CM5 Sensor Chip can Human PD-1 Protein with an affinity constant of 1.0-5.0 μM as determined in a SPR assay.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit proliferation of HT-29 cells. The ED50 this effect is 0.1452 μg/mL, corresponding to a specific activity is 6.89×103 units/mg

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized PD-1 Protein, Human can bind IBI-363 (HY-P991740). The ED50 for this effect is 4.714 ng/mL.

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded Pembrolizumab (HY-P9902) on AHC2 biosensor, can bind PD-1 Protein, Human (HEK293, His) with an affinity constant of 1.898E-09 M as determined in BLI assay.

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded Ivonescimab (HY-P99675) on AHC2 biosensor, can bind PD-1 Protein, Human (HEK293, His) with an affinity constant of 5.804E-08 M as determined in BLI assay.

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded Rosnilimab (HY-P9997) on AHC2 biosensor, can bind PD-1 Protein, Human (HEK293, His) with an affinity constant of 2.896E-09 M as determined in BLI assay.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q15116 (L25-Q167)

  • Gene ID
    5133  [NCBI]
  • Molecular Construction
    • N-term
    • PD-1 (L25-Q167)
      Accession # Q15116
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    PDCD1; Systemic Lupus Erythematosus Susceptibility 2; Prev. SLEB2; HPD-1; PD1; Programmed Cell Death 1 Protein; Programmed Cell Death Protein 1; CD279 Antigen; CD279; ADMIO4; HSLE1; AIMTBS; PD-1; HPD-L; Programmed Cell Death 1; Protein PD-1

  • AA Sequence

    LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ

  • Molecular Weight

    Approximately 25-41 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, 5% trehalose, mannitol.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Syn NL, et al. De-novo and acquired resistance to immune checkpoint targeting. Lancet Oncol. 2017 Dec;18(12):e731-e741. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit proliferation of HT-29 cells. The ED50 this effect is 0.1452 μg/mL, corresponding to a specific activity is 6.89×103 units/mg

  • 1/16

    MedChemExpress Validation
     Immobilized PD-1 Protein, Human can bind IBI-363 (HY-P991740). The ED50 for this effect is 4.714 ng/mL.

  • 1/16

    MedChemExpress Validation
     Loaded Pembrolizumab (HY-P9902) on AHC2 biosensor, can bind PD-1 Protein, Human (HEK293, His) with an affinity constant of 1.898E-09 M as determined in BLI assay.

  • 1/16

    MedChemExpress Validation
     Loaded Ivonescimab (HY-P99675) on AHC2 biosensor, can bind PD-1 Protein, Human (HEK293, His) with an affinity constant of 5.804E-08 M as determined in BLI assay.

  • 1/16

    MedChemExpress Validation
     Loaded Rosnilimab (HY-P9997) on AHC2 biosensor, can bind PD-1 Protein, Human (HEK293, His) with an affinity constant of 2.896E-09 M as determined in BLI assay.

  • PD-1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for PD-1 Protein, Human (HEK293, His)
  • Experimental Validation Results for PD-1 Protein, Human (HEK293, His)
  • Experimental Validation Results for PD-1 Protein, Human (HEK293, His)
  • Experimental Validation Results for PD-1 Protein, Human (HEK293, His)
  • Experimental Validation Results for PD-1 Protein, Human (HEK293, His)
  • Experimental Validation Results for PD-1 Protein, Human (HEK293, His)
  • PD-1 Protein, Human (HEK293, His) Product Image