PDGF R beta Protein, Rhesus Macaque (HEK293, His)

PDGF R beta, a tyrosine-protein kinase receptor, crucially regulates embryonic development, overseeing cell processes like proliferation, survival, differentiation, chemotaxis, and migration. Especially vital in blood vessel development, it facilitates pericyte and smooth muscle cell proliferation, migration, and recruitment to endothelial cells, playing a significant role in blood vessel formation and maintenance. PDGF R beta Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived PDGF R beta protein, expressed by HEK293 , with C-His labeled tag.

PDGF R beta, a tyrosine-protein kinase, serves as a cell-surface receptor for homodimeric PDGFB and PDGFD, as well as heterodimers formed by PDGFA and PDGFB. This receptor plays a crucial role in the intricate regulation of embryonic development, overseeing processes such as cell proliferation, survival, differentiation, chemotaxis, and migration. Particularly vital in blood vessel development, PDGF R beta facilitates the proliferation, migration, and recruitment of pericytes and smooth muscle cells to endothelial cells, contributing significantly to the formation and maintenance of blood vessels.

1.This product does not contain protein kinase domain.
2.Measured by its ability to inhibit the biological activity of PDGF-BB using NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 2.236 µg/mL in the presence of 50 ng/mL Recombinant Human PDGF-BB, corresponding to a specific activity is 447.22 Unit/mg.

• 
  Measured by its ability to inhibit the biological activity of PDGF-BB using NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 2.236 µg/mL in the presence of 50 ng/mL Recombinant Human PDGF-BB, corresponding to a specific activity is 447.22 unit/mg.

Rhesus Macaque

HEK293

C-His

F7BTH1 (L33-F530)

711353  [NCBI]

Partial

LVITPPGPELILNVSSTFVLTCSGSAPVVWERMSQELPQEMAKAQDNTFSSVLTLTNLTGLDTGEYFCTYNDSRGLEPDERKRLYIFVPDPTMGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDIALPVPYDHQRGFSGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWMYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGALQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPMLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDMQEVIVVPHSLPF

Approximately 80-96 kDa.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.