PRAK/MAPKAPK5 Protein, Human (sf9, His-GST)

PRAK/MAPKAPK5 protein, a serine/threonine kinase, responds to cellular stress and pro-inflammatory cytokines and acts as a tumor suppressor through MAP kinases (including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta) activated by phosphorylation. Initially located in the nucleus, upon activation, it moves to the cytoplasm and phosphorylates the heat shock protein HSP27. PRAK/MAPKAPK5 Protein, Human (sf9, His-GST) is the recombinant human-derived PRAK/MAPKAPK5 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of PRAK/MAPKAPK5 Protein, Human (sf9, His-GST) is 471 a.a., with molecular weight of ~35-45 kDa.

The PRAK/MAPKAPK5 Protein, belonging to the serine/threonine kinase family, functions as a tumor suppressor and responds to cellular stress and proinflammatory cytokines by getting activated through phosphorylation by MAP kinases, including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. While initially located in the nucleus, upon phosphorylation and activation, it translocates to the cytoplasm. A notable target of this kinase is the heat shock protein HSP27, phosphorylating it at physiologically relevant sites. This gene displays two alternatively spliced transcript variants encoding distinct isoforms. PRAK/MAPKAPK5 exhibits ubiquitous expression, with discernible levels in the small intestine (RPKM 3.1), colon (RPKM 2.9), and 25 other tissues, emphasizing its widespread involvement in diverse physiological contexts across multiple organs.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

C-Strep

Q8IW41-2/NP_003659.2 (M1-Q471)

8550  [NCBI]

Approximately 35-45 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.