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R-Cadherin/CDH4 Protein, Human (HEK293, His)

Cat. No.: HY-P77172
COA Handling Instructions

The R-Cadherin/CDH4 protein facilitates cell adhesion through homophilic interactions, aiding in cell connections and potential cell type sorting. It may play a crucial role in retinal development, indicating its significance in orchestrating cellular processes within the context of eye development and function. R-Cadherin/CDH4 Protein, Human (HEK293, His) is the recombinant human-derived R-Cadherin/CDH4 protein, expressed by HEK293 , with C-His labeled tag. The total length of R-Cadherin/CDH4 Protein, Human (HEK293, His) is 714 a.a., with molecular weight of 90-100 kDa.

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Description

The R-Cadherin/CDH4 protein facilitates cell adhesion through homophilic interactions, aiding in cell connections and potential cell type sorting. It may play a crucial role in retinal development, indicating its significance in orchestrating cellular processes within the context of eye development and function. R-Cadherin/CDH4 Protein, Human (HEK293, His) is the recombinant human-derived R-Cadherin/CDH4 protein, expressed by HEK293 , with C-His labeled tag. The total length of R-Cadherin/CDH4 Protein, Human (HEK293, His) is 714 a.a., with molecular weight of 90-100 kDa.

Background

R-Cadherin, or CDH4, is a calcium-dependent cell adhesion protein that facilitates cellular interactions through homophilic binding, primarily interacting with itself. This characteristic enables cadherins to play a crucial role in the cohesion of connecting cells, potentially contributing to the sorting of diverse cell types. The significance of R-Cadherin extends to retinal development, where its involvement suggests a pivotal function in the intricate processes that govern the formation and organization of the retina. By participating in calcium-dependent cell adhesion, R-Cadherin emerges as a key player in the regulation of cellular arrangements and tissue development, particularly in the context of retinal tissue.

Biological Activity

Measured in a cell proliferation assay using SH-SY5Y cells. The ED50 for this effect is 0.3577 μg/mL, corresponding to a specific activity is 2.796×104 units/mg.

Species

Human

Source

HEK293

Tag

C-His

Accession

P55283 (H21-A734)

Gene ID
Molecular Construction
N-term
CDH4 (H21-A734)
Accession # P55283
His
C-term
Synonyms
Cadherin-4; Retinal cadherin; R-CAD; R-cadherin
AA Sequence

HNEDLTTRETCKAGFSEDDYTALISQNILEGEKLLQVKFSSCVGTKGTQYETNSMDFKVGADGTVFATRELQVPSEQVAFTVTAWDSQTAEKWDAVVRLLVAQTSSPHSGHKPQKGKKVVALDPSPPPKDTLLPWPQHQNANGLRRRKRDWVIPPINVPENSRGPFPQQLVRIRSDKDNDIPIRYSITGVGADQPPMEVFSIDSMSGRMYVTRPMDREEHASYHLRAHAVDMNGNKVENPIDLYIYVIDMNDNRPEFINQVYNGSVDEGSKPGTYVMTVTANDADDSTTANGMVRYRIVTQTPQSPSQNMFTINSETGDIVTVAAGLDREKVQQYTVIVQATDMEGNLNYGLSNTATAIITVTDVNDNPPEFTASTFAGEVPENRVETVVANLTVMDRDQPHSPNWNAVYRIISGDPSGHFSVRTDPVTNEGMVTVVKAVDYELNRAFMLTVMVSNQAPLASGIQMSFQSTAGVTISIMDINEAPYFPSNHKLIRLEEGVPPGTVLTTFSAVDPDRFMQQAVRYSKLSDPASWLHINATNGQITTAAVLDRESLYTKNNVYEATFLAADNGIPPASGTGTLQIYLIDINDNAPELLPKEAQICEKPNLNAINITAADADVDPNIGPYVFELPFVPAAVRKNWTITRLNGDYAQLSLRILYLEAGMYDVPIIVTDSGNPPLSNTSIIKVKVCPCDDNGDCTTIGAVAAAGLGTGA

Molecular Weight

Approximately 90-115 kDa

Purity

Greater than 85% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 100 mM NaCl, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

R-Cadherin/CDH4 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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R-Cadherin/CDH4 Protein, Human (HEK293, His)
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