RSPO2/R-Spondin 2 Protein, Human (HEK293, Fc)

RSPO2, a pivotal activator of the canonical Wnt pathway, acts as a ligand for LGR4-6 receptors, forming a complex with phosphorylated LRP6 and frizzled receptors. This interaction activates the canonical Wnt pathway, increasing target gene expression. RSPO2 also regulates both canonical and non-canonical Wnt signaling by inhibiting ZNRF3. During embryonic development, RSPO2 plays a crucial role in limb specification by amplifying the Wnt pathway independently of LGR4-6 receptors, possibly by acting as an antagonistic ligand to RNF43 and ZNRF3. RSPO2 interacts with WNT1, binds heparin, and engages with LGR4, LGR5, LGR6, RNF43, and ZNRF3, highlighting its involvement in the intricate regulatory network of the Wnt pathway. RSPO2/R-Spondin 2 Protein, Human (HEK293, Fc) is the recombinant human-derived RSPO2/R-Spondin 2 protein, expressed by HEK293, with C-hFc labeled tag. The total length of RSPO2/R-Spondin 2 Protein, Human (HEK293, Fc) is 205 a.a., with molecular weight of ~50 kDa.

RSPO2, a pivotal activator of the canonical Wnt signaling pathway, functions as a ligand for LGR4-6 receptors, particularly LGR4, LGR5, or LGR6. Upon binding to these receptors, the resultant complex associates with phosphorylated LRP6 and frizzled receptors, which are activated by extracellular Wnt receptors. This interaction triggers the canonical Wnt signaling pathway, leading to the increased expression of target genes. Additionally, RSPO2 serves as a regulator of both the canonical Wnt/beta-catenin-dependent pathway and the non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, a crucial regulator of the Wnt signaling pathway. Notably, during embryonic development, RSPO2 plays a crucial role in limb specification by amplifying the Wnt signaling pathway independently of LGR4-6 receptors. This amplification occurs, possibly by RSPO2 acting as a direct antagonistic ligand to RNF43 and ZNRF3, thereby governing the number of limbs an embryo should form. RSPO2 interacts with WNT1, binds heparin, and interacts with LGR4, LGR5, and LGR6. Furthermore, RSPO2 engages with the E3 ubiquitin ligases RNF43 and ZNRF3, indicating its involvement in the intricate regulatory network of the Wnt signaling pathway.

Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells and the ED₅₀ is typically 2-12 ng/mL in the presence of 5 ng/mL mouse Wnt3a.

Human

HEK293

C-hFc

Q6UXX9 (M1-G205)

340419  [NCBI]

Approximately 50 kDa

Greater than 90% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of Histidine, Arginine, 120 mM NaCl, 0.02% Tween 80, 5% sucrose, pH 6.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.