RSPO2/R-spondin-2 Protein, Mouse (Fc)

RSPO2, a key activator of the canonical Wnt pathway, acts as a ligand for LGR4-6 receptors (LGR4, LGR5, or LGR6). Upon binding, the complex forms with phosphorylated LRP6 and frizzled receptors, activating the canonical Wnt pathway and upregulating target genes. RSPO2 also regulates both canonical and non-canonical Wnt signaling by inhibiting ZNRF3. Crucially, during embryonic development, RSPO2 plays a vital role in limb specification by independently amplifying the Wnt pathway, possibly through direct antagonistic interactions with RNF43 and ZNRF3, influencing limb formation. Interactions with WNT1, heparin, and LGR4, LGR5, and LGR6 highlight RSPO2's complex role in Wnt signaling regulation. RSPO2/R-spondin-2 Protein, Mouse (Fc) is the recombinant mouse-derived RSPO2/R-spondin-2 protein, expressed by HEK293, with C-mFc labeled tag. The total length of RSPO2/R-spondin-2 Protein, Mouse (Fc) is 182 a.a., with molecular weight of ~47.4 kDa.

RSPO2, a pivotal activator of the canonical Wnt signaling pathway, serves as a ligand for LGR4-6 receptors, namely LGR4, LGR5, or LGR6. Upon binding to these receptors, the formed complex associates with phosphorylated LRP6 and frizzled receptors activated by extracellular Wnt receptors, thereby initiating the canonical Wnt signaling pathway and leading to increased expression of target genes. Additionally, RSPO2 acts as a regulator of both the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by inhibiting ZNRF3, a key regulator of the Wnt signaling pathway. This multifaceted protein may also function as a ligand for frizzled and LRP receptors. Notably, during embryonic development, RSPO2 plays a crucial role in limb specification by amplifying the Wnt signaling pathway independently of LGR4-6 receptors, potentially by acting as a direct antagonistic ligand to RNF43 and ZNRF3, thus influencing the formation of limbs in embryos. Interactions with WNT1, heparin, and LGR4, LGR5, and LGR6 underscore the complexity of RSPO2's involvement in Wnt signaling regulation.

Mouse

HEK293

C-mFc

Q8BFU0 (N24-G205)

239405  [NCBI]

Approximately 47.4 kDa

Greater than 85% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.