Serglycin/SRGN Protein, Human (HEK293, His-Myc)

Serine (SRGN) is essential for the formation of mast cell secretory granules and the storage of various compounds. It plays a crucial role in the storage of proteases in mast cells, including granzyme B in T lymphocytes, and contributes to the localization of neutrophil elastase. Serglycin/SRGN Protein, Human (HEK293, His-Myc) is the recombinant human-derived Serglycin/SRGN protein, expressed by HEK293 , with C-His, C-Myc labeled tag. The total length of Serglycin/SRGN Protein, Human (HEK293, His-Myc) is 158 a.a., with molecular weight of ~27 kDa.

Serglycin (SRGN) serves as a crucial player in the formation of mast cell secretory granules, facilitating the storage of various compounds within secretory vesicles. Its indispensability is underscored by its role in storing proteases in connective tissue and mucosal mast cells, including the storage of granzyme B in T-lymphocytes. SRGN also contributes to the localization of neutrophil elastase in azurophil granules of neutrophils. Furthermore, it participates in the processing of MMP2 and, notably, plays a key role in granule-mediated apoptosis by forming a complex with granzyme B. This complex is delivered to cells by perforin, inducing apoptosis. Beyond its involvement in cytotoxic cell granule-mediated processes, SRGN regulates the secretion of TNF-alpha and potentially influences protease secretion. Intriguingly, SRGN exhibits inhibitory effects on bone mineralization and engages in binding interactions with activated CD44 and granzyme B (GZMB), highlighting its multifaceted roles in cellular processes and immune regulation.

Human

HEK293

C-His;C-Myc

P10124 (M1-L158)

5552  [NCBI]

Approximately 27 kDa

Greater than 90% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.