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Serpin B3 Protein, Human (HEK293, His)

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The Serpin B3 protein exhibits versatility in cellular regulation and may act as a papain-like cysteine protease inhibitor to modulate immune responses against tumor cells. Its dual role extends to inhibiting UV-induced apoptosis by inhibiting c-Jun NH(2)-terminal kinase (JNK1) activity, suggesting that Serpin B3 is involved in the stress response. Serpin B3 Protein, Human (HEK293, His) is the recombinant human-derived Serpin B3 protein, expressed by HEK293 , with C-6*His labeled tag.

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  • Species: Human
  • Source: HEK293
  • 保管条件:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Serpin B3 Protein, Human (HEK293, His) Product Image
  • Serpin B3 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
最寄りの代理店をお探しください Your local distributor: Hycultec GmbHHycultec
  • 生物活性
  • Technical Parameters
  • Product Properties
  • ドキュメント
  • Help & FAQs
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生物活性

製品説明

The Serpin B3 protein exhibits versatility in cellular regulation and may act as a papain-like cysteine protease inhibitor to modulate immune responses against tumor cells. Its dual role extends to inhibiting UV-induced apoptosis by inhibiting c-Jun NH(2)-terminal kinase (JNK1) activity, suggesting that Serpin B3 is involved in the stress response. Serpin B3 Protein, Human (HEK293, His) is the recombinant human-derived Serpin B3 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The Serpin B3 protein emerges as a versatile participant in cellular regulation, potentially functioning as a papain-like cysteine protease inhibitor to modulate the host immune response against tumor cells. Its dual role extends to the inhibition of UV-induced apoptosis, accomplished by suppressing the activity of c-Jun NH(2)-terminal kinase (JNK1), thereby implicating Serpin B3 in cellular processes related to stress response. The protein's interaction with MAPK8/JNK1 further underscores its intricate involvement in signaling pathways, highlighting its potential as a multifaceted regulator with implications for immune modulation and apoptosis regulation.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • アクセッション番号

    P29508 (M1-P390)

  • 遺伝子ID
    6317  [NCBI]
  • Molecular Construction
    • N-term
    • Serpin B3 (M1-P390)
      Accession # P29508
    • 6*His
    • C-term
  • Protein Length

    Full Length

  • 別名

    Serpin B3; Protein T4-A; Squamous cell carcinoma antigen 1; SCCA-1; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3; serpin peptidase inhibitor, clade B (ovalbumin), member 3; Squamous cell carcinoma antigen 1; T4-A; SCCA1

  • AA Sequence

    MNSLSEANTKFMFDLFQQFRKSKENNIFYSPISITSALGMVLLGAKDNTAQQIKKVLHFDQVTENTTGKAATYHVDRSGNVHHQFQKLLTEFNKSTDAYELKIANKLFGEKTYLFLQEYLDAIKKFYQTSVESVDFANAPEESRKKINSWVESQTNEKIKNLIPEGNIGSNTTLVLVNAIYFKGQWEKKFNKEDTKEEKFWPNKNTYKSIQMMRQYTSFHFASLEDVQAKVLEIPYKGKDLSMIVLLPNEIDGLQKLEEKLTAEKLMEWTSLQNMRETRVDLHLPRFKVEESYDLKDTLRTMGMVDIFNGDADLSGMTGSRGLVLSGVLHKAFVEVTEEGAEAAAATAVVGFGSSPTSTNEEFHCNHPFLFFIRQNKTNSILFYGRFSSP

  • Predicted Molecular Mass

    46 kDa

  • 分子量

    Approximately 41-50 kDa, based on SDS-PAGE under reducing conditions.

  • 純度

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

性状

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 0.02% Tween80, 4% Mannitol, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

輸送条件

Room temperature in continental US; may vary elsewhere.

Calculators

再構成計算機

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial)
=
Mass (in vial)
÷
Desired Reconstitution Concentration
希釈計算機

濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)

濃度 (開始)
×
体積 (開始)
=
濃度 (終了)
×
体積 (終了)
比活性計算機
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50)
106 ÷
ng/mL

Serpin B3 Protein, Human (HEK293, His) 関連分類

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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