Siglec-2/CD22 Protein, Mouse (Biotinylated, HEK293, His)
Siglec-2/CD22 is a B cell surface receptor that mediates B cell interactions and is involved in B cell localization within lymphoid tissues. It selectively binds α-2,6-linked sialic acids, including CD45, and its sialic acid recognition site may be regulated by cis interactions. Siglec-2/CD22 Protein, Mouse (Biotinylated, HEK293, His) is the recombinant mouse-derived Siglec-2/CD22 protein, expressed by HEK293 , with His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
Siglec-2/CD22 is a B cell surface receptor that mediates B cell interactions and is involved in B cell localization within lymphoid tissues. It selectively binds α-2,6-linked sialic acids, including CD45, and its sialic acid recognition site may be regulated by cis interactions. Siglec-2/CD22 Protein, Mouse (Biotinylated, HEK293, His) is the recombinant mouse-derived Siglec-2/CD22 protein, expressed by HEK293 , with His labeled tag.
Background
Siglec-2/CD22 protein functions as a mediator of B-cell interactions and is potentially involved in the localization of B-cells within lymphoid tissues. This protein selectively binds to sialylated glycoproteins, with a preference for alpha-2,6-linked sialic acid, and one of its binding partners is CD45. The recognition site for sialic acid can be masked through cis interactions with sialic acids on the same cell surface. Upon ligand-induced tyrosine phosphorylation during immune responses, Siglec-2/CD22 is implicated in the regulation of B-cell antigen receptor signaling. It plays a dual role in signaling, positively regulating interactions with Src family tyrosine kinases and potentially acting as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains. This recruitment blocks signal transduction through the dephosphorylation of signaling molecules. In molecular interactions, Siglec-2/CD22 associates with proteins such as LYN, SYK, PIK3R1/PIK3R2, PLCG1, SHC1, INPP5D, and GRB2 upon phosphorylation, suggesting its involvement in complex signaling networks that modulate B-cell responses.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag His
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Accession
P35329-1 (S22-P702)
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Gene ID12483
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Protein Length
Partial Extracellular Domain
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Conjugation
Biotin
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Synonyms
CD22; B-Lymphocyte Cell Adhesion Molecule; CD22 Molecule; T-Cell Surface Antigen Leu-14; SIGLEC2; BL-CAM; Sialic Acid-Binding Ig-Like Lectin 2; Sialic Acid Binding Ig-Like Lectin 2; B-Cell Receptor CD22; CD22 Protein; CD22 Antigen; Siglec-2; SIGLEC-2
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AA Sequence
SANDWTVDHPQTLFAWEGACIRIPCKYKTPLPKARLDNILLFQNYEFDKATKKFTGTVLYNATKTEKDPESELYLSKQGRVTFLGNRIDNCTLKIHPIRANDSGNLGLRMTAGTERWMEPIHLNVSEKPFQPYIQMPSEIRESQSVTLTCGLNFSCFGYDILLKWFLEDSEITSITSSVTSITSSVTSSIKNVYTESKLTFQPKWTDHGKSVKCQVQHSSKVLSERTVRLDVKYTPKLEIKVNPTEVEKNNSVTMTCRVNSSNPKLRTVAVSWFKDGRPLEDQELEQEQQMSKLILHSVTKDMRGKYRCQASNDIGPGESEEVELTVHYAPEPSRVHIYPSPAEEGQSVELICESLASPSATNYTWYHNRKPIPGDTQEKLRIPKVSPWHAGNYSCLAENRLGHGKIDQEAKLDVHYAPKAVTTVIQSFTPILEGDSVTLVCRYNSSNPDVTSYRWNPQGSGSVLKPGVLRIQKVTWDSMPVSCAACNHKCSWALPVILNVHYAPRDVKVLKVSPASEIRAGQRVLLQCDFAESNPAEVRFFWKKNGSLVQEGRYLSFGSVSPEDSGNYNCMVNNSIGETLSQAWNLQVLYAPRRLRVSISPGDHVMEGKKATLSCESDANPPISQYTWFDSSGQDLHSSGQKLRLEPLEVQHTGSYRCKGTNGIGTGESPPSTLTVYYSP
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Predicted Molecular Mass
78.3 kDa
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Molecular Weight
Approximately 90-110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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