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  5. Siglec-8 Protein, Human (HEK293, Fc)

Siglec-8 Protein, Human (HEK293, Fc)

Cat. No.: HY-P71312
Handling Instructions

Siglec-8 protein is a putative adhesion molecule that mediates sialic acid-dependent binding to erythrocytes, preferring α-2,3-linked sialic acid and interacting with α-2,6-linked sialic acid . Its sialic acid recognition site may be hidden through cis interactions with sialic acid on the surface of the same cell. Siglec-8 Protein, Human (HEK293, Fc) is the recombinant human-derived Siglec-8 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Siglec-8 Protein, Human (HEK293, Fc) is 347 a.a., with molecular weight of 80-100 kDa.

For research use only. We do not sell to patients.

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Description

Siglec-8 protein is a putative adhesion molecule that mediates sialic acid-dependent binding to erythrocytes, preferring α-2,3-linked sialic acid and interacting with α-2,6-linked sialic acid . Its sialic acid recognition site may be hidden through cis interactions with sialic acid on the surface of the same cell. Siglec-8 Protein, Human (HEK293, Fc) is the recombinant human-derived Siglec-8 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Siglec-8 Protein, Human (HEK293, Fc) is 347 a.a., with molecular weight of 80-100 kDa.

Background

Siglec-8 protein, identified as a putative adhesion molecule, serves as a mediator for sialic-acid dependent binding to red blood cells. It exhibits a preference for binding to alpha-2,3-linked sialic acid and also interacts with alpha-2,6-linked sialic acid. Notably, the sialic acid recognition site of Siglec-8 may be concealed due to cis interactions with sialic acids present on the same cell surface. Furthermore, it has the ability to simultaneously recognize epitopes featuring a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose, with a specific affinity for Gal-6-sulfated sialyl-Lewis X glycan epitopes.

Species

Human

Source

HEK293

Tag

C-hFc

Accession

Q9NYZ4 (M17-A363)

Gene ID
Molecular Construction
N-term
Siglec-8 (M17-A363)
Accession # Q9NYZ4
hFc
C-term
Synonyms
Siglec8; Siglec-8; SAF2; SAF2SAF-2; SAF-2; CD329 antigen; CDw329
AA Sequence

MEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTALTHRPDILILGTLESGHSRNLTCSVPWACKQGTPPMISWIGASVSSPGPTTARSSVLTLTPKPQDHGTSLTCQVTLPGTGVTTTSTVRLDVSYPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGTGTSRPVSQVTLAA

Molecular Weight

80-100 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Siglec-8 Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Siglec-8 Protein, Human (HEK293, Fc)
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HY-P71312
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