2. Recombinant Proteins
  3. Cytokines and Growth Factors CD Antigens Receptor Proteins Enzymes & Regulators
  4. Angiopoietins Endothelial cell CD Proteins Receptor Tyrosine Kinases Protein Tyrosine Kinases
  5. TIE-2/CD202b TIE Receptors
  6. TIE-2
  7. TIE-2 Protein, Human (HEK293, His-Fc)

TIE-2 Protein, Human (HEK293, His-Fc)

Cat. No.: HY-P73434
Handling Instructions

TIE2 Protein is a cell surface receptor for ANGPT1, ANGPT2, and ANGPT4, belonging to the protein tyrosine kinase Tie2 family. TIE2 Protein is an angiogenic factor that regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion, cell spreading, and actin cytoskeleton reorganization, and has anti-inflammatory effects. TIE2 Protein consists of 1124 amino acids (M1-A1124), with two immunoglobulin-like domains, three N-terminal fibronectin type III domains (447-541 a.a, 545-636 a.a, 641-735 a.a), and three epidermal growth factor (EGF)-like domains (210-252 a.a, 254-299 a.a, 301-341 a.a). TIE-2 Protein, Human (HEK293, His-Fc) is the recombinant human-derived TIE-2 protein, expressed by HEK293 , with C-hFc, C-His labeled tag. The total length of TIE-2 Protein, Human (HEK293, His-Fc) is 723 a.a., with molecular weight of ~130.7 kDa.

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Description

TIE2 Protein is a cell surface receptor for ANGPT1, ANGPT2, and ANGPT4, belonging to the protein tyrosine kinase Tie2 family. TIE2 Protein is an angiogenic factor that regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion, cell spreading, and actin cytoskeleton reorganization, and has anti-inflammatory effects. TIE2 Protein consists of 1124 amino acids (M1-A1124), with two immunoglobulin-like domains, three N-terminal fibronectin type III domains (447-541 a.a, 545-636 a.a, 641-735 a.a), and three epidermal growth factor (EGF)-like domains (210-252 a.a, 254-299 a.a, 301-341 a.a). TIE-2 Protein, Human (HEK293, His-Fc) is the recombinant human-derived TIE-2 protein, expressed by HEK293 , with C-hFc, C-His labeled tag. The total length of TIE-2 Protein, Human (HEK293, His-Fc) is 723 a.a., with molecular weight of ~130.7 kDa.
Background

TIE2 Protein binds to angiogenin-1 and mediates signaling pathways that play a role in embryonic vascular development. TIE2 Protein has anti-inflammatory effects by regulating the activity of pro-inflammatory plasma proteins and leukocytes from the blood vessels. TIE2 Protein regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion, and cell spreading, as well as the reorganization of the actin cytoskeleton. The expression of TIE2 Protein can promote tumor angiogenesis and accelerate tumor growth and metastasis[1][2].
Species

Human
Source

HEK293
Tag

C-hFc;C-His
Accession

AAA61139.1 (A23-K745)
Gene ID
Molecular Construction
N-term
TIE-2 (A23-K745)
Accession # AAA61139.1
hFc-His
C-term
Synonyms
Angiopoietin-1 receptor; CD202b; hTIE2; p140 TEK; Tie2; VMCM
Molecular Weight

Approximately 130.7 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

TIE-2 Protein, Human (HEK293, His-Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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