VE-Cadherin Protein, Human (HEK293, C-His-Fc)

The FGFR-4 protein binds to its ligands and activates signaling pathways involved in cell growth, differentiation, and survival. It plays a role in various developmental processes, including skeletal development, liver metabolism, and cancer progression. In the absence of ligand binding, FGFR-4 remains inactive and does not initiate downstream signaling cascades. VE-Cadherin Protein, Human (HEK293, C-His-Fc) is the recombinant human-derived VE-Cadherin protein, expressed by HEK293 , with C-hFc, C-6*His labeled tag. The total length of VE-Cadherin Protein, Human (HEK293, C-His-Fc) is 568 a.a., with molecular weight of ~125 kDa.

The VE-Cadherin Protein, belonging to the classical cadherin superfamily, encodes a preproprotein that undergoes proteolytic processing to yield the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule comprises five extracellular cadherin repeats, a transmembrane region, and a highly conserved cytoplasmic tail. Operating as a classical cadherin, it confers upon cells the ability to adhere in a homophilic manner, thereby playing a pivotal role in the assembly and maintenance of endothelial adherens junctions. Located in a gene cluster on the long arm of chromosome 16, this gene is implicated in loss of heterozygosity events in breast and prostate cancer. Noteworthy is its biased expression, with elevated levels observed in the placenta (RPKM 108.8), lung (RPKM 57.5), and 12 other tissues, underscoring its potential significance across a spectrum of physiological contexts.

Measured by the ability of the immobilized protein to support the adhesion of HUVEC cells. The ED₅₀ for this effect is 59.22 ng/ml, corresponding to a specific activity is 1.689×10^4 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of HUVEC cells. The ED₅₀ for this effect is 59.22 ng/mL, corresponding to a specific activity is 1.689×10⁴ units/mg.

Human

HEK293

C-hFc;C-6*His

NP_001786.2 (A26-Q593)

1003  [NCBI]

AGANPAQRDTHSLLPTHRRQKRDWIWNQMHIDEEKNTSLPHHVGKIKSSVSRKNAKYLLKGEYVGKVFRVDAETGDVFAIERLDRENISEYHLTAVIVDKDTGENLETPSSFTIKVHDVNDNWPVFTHRLFNASVPESSAVGTSVISVTAVDADDPTVGDHASVMYQILKGKEYFAIDNSGRIITITKSLDREKQARYEIVVEARDAQGLRGDSGTATVLVTLQDINDNFPFFTQTKYTFVVPEDTRVGTSVGSLFVEDPDEPQNRMTKYSILRGDYQDAFTIETNPAHNEGIIKPMKPLDYEYIQQYSFIVEATDPTIDLRYMSPPAGNRAQVIINITDVDEPPIFQQPFYHFQLKENQKKPLIGTVLAMDPDAARHSIGYSIRRTSDKGQFFRVTKKGDIYNEKELDREVYPWYNLTVEAKELDSTGTPTGKESIVQVHIEVLDENDNAPEFAKPYQPKVCENAVHGQLVLQISAIDKDITPRNVKFKFILNTENNFTLTDNHDNTANITVKYGQFDREHTKVHFLPVVISDNGMPSRTGTSTLTVAVCKCNEQGEFTFCEDMAAQ

Approximately 125 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.