VEGF-CC Protein, Mouse/Rat (HEK293, Fc)

VEGF-CC protein stimulates cell proliferation, migration, and vascular permeability, which are critical for angiogenesis and endothelial cell dynamics. It plays a crucial role in the development of the venous and lymphatic vasculature during embryogenesis and in the maintenance of adult lymphatic endothelium. VEGF-CC Protein, Mouse/Rat (HEK293, Fc) is the recombinant rat, mouse-derived VEGF-CC protein, expressed by HEK293 , with N-hFc labeled tag. The total length of VEGF-CC Protein, Mouse/Rat (HEK293, Fc) is 116 a.a., with molecular weight of ~44 & 34 kDa, respectively.

VEGF-CC, a growth factor crucial in angiogenesis and endothelial cell dynamics, exerts stimulatory effects on cellular proliferation and migration, while also influencing the permeability of blood vessels. It plays a vital role in angiogenesis, particularly in the development of the venous and lymphatic vascular systems during embryogenesis. Additionally, VEGF-CC contributes to the maintenance of differentiated lymphatic endothelium in adults. The protein binds and activates the KDR/VEGFR2 and FLT4/VEGFR3 receptors, orchestrating essential signaling pathways for vascular development and homeostasis. Structurally, VEGF-CC forms a homodimer with a non-covalent and antiparallel arrangement. Its interaction with FLT4/VEGFR3 is imperative for FLT4/VEGFR3 homodimerization and subsequent activation, highlighting the intricacies of its regulatory role in vascular processes.

1.Immobilized mouse FLT4-His at 10 μg/mL (100 μL/well) can bind mouse/rat Fc-VEGFC and the EC₅₀ is 0.41-0.95 μg/mL.
2.Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) and the ED₅₀ is typically 70-300 ng/mL

Rat; Mouse

HEK293

N-hFc

P97953 (A108-R223)/O35757(A108-R223)

22341  [NCBI]/114111

Approximately 44&34 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.