VISTA/B7-H5 Protein, Human (Biotinylated, HEK293, Fc-Avi)

VISTA/B7-H5 protein, an immunoregulatory receptor, inhibits T-cell response and may regulate embryonic stem cell differentiation by inhibiting BMP4 signaling. It also stimulates MMP14-mediated MMP2 activation, suggesting a role in matrix metalloproteinase processes. These roles highlight the significance of VISTA/B7-H5 in immune regulation, embryonic development, and extracellular matrix dynamics. VISTA/B7-H5 Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived VISTA/B7-H5 protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag. The total length of VISTA/B7-H5 Protein, Human (Biotinylated, HEK293, Fc-Avi) is 162 a.a., with molecular weight of 55-66 kDa.

VISTA/B7-H5 protein, functioning as an immunoregulatory receptor, plays a pivotal role in inhibiting the T-cell response, as established in various studies. Additionally, it may contribute to the differentiation of embryonic stem cells by inhibiting BMP4 signaling, showcasing its potential role in developmental processes. Moreover, VISTA/B7-H5 has been implicated in stimulating MMP14-mediated MMP2 activation, suggesting a regulatory function in matrix metalloproteinase-mediated processes. This multifaceted role underscores the significance of VISTA/B7-H5 in immune regulation, embryonic development, and extracellular matrix dynamics, revealing its potential impact across diverse biological contexts.

Human

HEK293

C-Avi;C-hFc

Q9H7M9 (F33-A194)

64115  [NCBI]

Extracellular Domain

Biotin

FKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQDSENITAA

Approximately 55-66 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of Tris with Glycine, Arginine and NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.