Subtilisin, bacillus licheniformis  (Synonyms: Bacillopeptidaseb)

Subtilisin (Compound proteinase) (EC 3.4.21.62) is a proteolytic enzyme, isolated from Bacillus licheniformis. Subtilisin (Compound proteinase) has catalytic activity in anhydrous dimethyl formamide. Subtilisin (Compound proteinase) can be used as a catalyst for easy coupling between sugars and amino acids^[1][2].

Product Information
This product is a proteolytic enzyme derived from Bacillus licheniformis 2709, which was obtained through mutagenic breeding of bacterial protoplasts. It is produced via deep fermentation, extraction, and refinement processes. The primary enzymatic component is Bacillus licheniformis protease, a serine-type endopeptidase that hydrolyzes peptide chains in protein molecules to produce polypeptides or amino acids. It exhibits strong protein-degrading capability. Optimal reaction conditions: temperature 50-55°C, pH 8.0-10.0.

Instructions
This product is water-soluble and can be dissolved in water or according to specific experimental literature references. It is susceptible to inhibition and damage by heavy metal ions (Fe³⁺, Cu²⁺, Hg⁺, Pb⁺, etc.) and oxidizing agents. Avoid contact with these substances during use and storage.

Subtilisin (Compound proteinase) (30 mg/mL; 2.5-7 d) selectively acylates disaccharides in anhydrous dimethylformamide^[1].
Subtilisin (Compound proteinase) (1.8 g; 20-72 h) selectively acylates monosaccharides in anhydrous dimethylformamide^[1].
Subtilisin (Compound proteinase) proteinase acylates D-glucose of flavanol isoquercetin (1) in the presence of activated ester trifluoroethyl butyrate (TFEB)^[3].
Subtilisin (Compound proteinase) improves the solubility of protein and fatty acids, and restores polypeptides and unsaturated fatty acids^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Subtilisin (Compound proteinase) hydrolysate (the ≤1 kDa meretrix lusoria protamex hydrolysate, MLPH) (150 mg/kg and 300 mg/kg; p.o.; once daily for 6 weeks) has anti-obesity and anti-hyperglycemic effects on ob/ob mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9014-01-1

Solid

Off-white to light yellow

[Subtilisin, bacillus licheniformis]

3.4.21.62

≥1000000 U/g

One unit is defined as the amount of enzyme that will hydrolysis of casein produced 1μg tyrosine per min at 40°C and pH 10.5.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Riva S, et al. Protease-catalyzed regioselective esterification of sugars and related compounds in anhydrous dimethylformamide[J]. Journal of the American Chemical Society, 1988, 110(2): 584-589.

  [2]. Philipp M, et al. Inhibition of subtilisin by substituted arylboronic acids. FEBS Lett. 1981 Oct 12;133(1):36-8.  [Content Brief]

  [3]. Danieli B, et al. On the regioselectivity of the protease subtilisin towards the acylation of enantiomeric pairs of benzyl and naphthyl glycopyranosides. Part 2[J]. Tetrahedron, 1999, 55(7): 2045-2060.

  [4]. Kim MJ, et al. Anti-Obesity and Anti-Hyperglycemic Effects of Meretrix lusoria Protamex Hydrolysate in ob/ob Mice. Int J Mol Sci. 2022 Apr 5;23(7):4015.  [Content Brief]